2'-O-Methylinosine
Based on 1 Customer Validation
2'-O-Methylinosine is a derivative of 2'-O-Methyladenosine. 2'-O-Methylinosine can be formed by chemical deamination of unmethylated 2'-O-Methyladenosine (HY-W011552). 2'-O-Methylinosine does not induce spore germination in Bacillus cereus. 2'-O-Methylinosine can be used in studies on diabetic nephropathy and HIV infection.
For research use only. We do not sell to patients.
- Purity : 99.27%
- CAS No.: 3881-21-8
- Formula: C11H14N4O5
- Molecular Weight:282.26
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Storage:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 3881-21-8
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Appearance Solid
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Molecular Weight 282.26
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Formula C11H14N4O5
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Color White to off-white
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SMILES
OC[C@@H]1[C@@H](O)[C@@H](OC)[C@H](N2C(N=CNC3=O)=C3N=C2)O1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (354.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.86 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.86 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[5]. Hyde RM. “Senseless” nucleic acid macromolecules: Synthesis, et al. The University of Utah; 2002.
[6]. Hyde RM, et al. Antiviral amphipathic oligo- and polyribonucleotides: analogue development and biological studies. Journal of medicinal chemistry. 2003 May 08;46(10):1878-85. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.5428 mL | 17.7142 mL | 35.4283 mL | 88.5708 mL |
| 5 mM | 0.7086 mL | 3.5428 mL | 7.0857 mL | 17.7142 mL | |
| 10 mM | 0.3543 mL | 1.7714 mL | 3.5428 mL | 8.8571 mL | |
| 15 mM | 0.2362 mL | 1.1809 mL | 2.3619 mL | 5.9047 mL | |
| 20 mM | 0.1771 mL | 0.8857 mL | 1.7714 mL | 4.4285 mL | |
| 25 mM | 0.1417 mL | 0.7086 mL | 1.4171 mL | 3.5428 mL | |
| 30 mM | 0.1181 mL | 0.5905 mL | 1.1809 mL | 2.9524 mL | |
| 40 mM | 0.0886 mL | 0.4429 mL | 0.8857 mL | 2.2143 mL | |
| 50 mM | 0.0709 mL | 0.3543 mL | 0.7086 mL | 1.7714 mL | |
| 60 mM | 0.0590 mL | 0.2952 mL | 0.5905 mL | 1.4762 mL | |
| 80 mM | 0.0443 mL | 0.2214 mL | 0.4429 mL | 1.1071 mL | |
| 100 mM | 0.0354 mL | 0.1771 mL | 0.3543 mL | 0.8857 mL |
2'-O-Methylinosine Related Classifications
HY-W013289 Related Classifications
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