Peptide Gross Weight Calculator
Calculate the theoretical net weight and the actual gross weight of peptide to use, based on concentration, volume, molecular weight, purity, and peptide content.
Net Weight (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
Gross Weight (g) = Net Weight (g) ÷ (Purity Percentage × Peptide Content Percentage)
1. Calculate the theoretical amount
2. Calculate the actual amount to use
Enter percentages from 0 to 100. Gross Weight = Net Weight ÷ [(Purity / 100) × (Peptide Content / 100)].
- 1. What about the amino acid abbreviations and general physicochemical properties?
- You may refer to our Amino Acid Reference Chart first. For special amino acids, please use the full name or include their chemical information in the remarks section if necessary.
- 2. What types of peptide modifications can MCE provide?
- Please refer to the modification list for details.
- 3. How to dissolve peptides?
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(1) Please refer to the product’s solubility testing results. If the report is not available, please contact us.
(2) You can also refer to our peptide solubility guidelines for details.
- 4. What kind of test reports are provided for custom peptides?
- The default analysis report includes HPLC, MS and COA.
- 5. What types of additional services can MCE provide?
- Elemental analysis, heavy metal testing, moisture determination, NMR and various other quality analyses can be provided for custom peptides. In addition, services such as aliquoting, TFA removal and salt exchange can be arranged upon request. Please specify the required analyses or services in the quotation if needed. Kindly contact us for any further questions.
- 6. What are the differences between various peptide salt forms, and how should they be selected?
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Common peptide salt forms include TFA salt, acetate salt, ammonium salt, hydrochloride salt, sodium salt and citrate salt. In general, salt formation does not affect the biological activity of peptides. TFA salt may theoretically exhibit some level of cytotoxicity, therefore its concentration should be considered when conducting cell-based or animal experiments. Acetate salt has minimal biological toxicity, leading to the preferred choice for biological studies. Whether desalted products are available depends on the specific peptide structure and desalting may affect peptide solubility.
By default, custom peptides are provided in TFA salt form. According to customers’ feedback, the influence of TFA in actual experiments is minimal. If desalting or salt exchange is required, please specify this in your quotation.
- 7. How to store peptides?
- It is recommended to store peptides at -20 °C or -80 °C. Fluorescent-labeled peptides should be kept away from light. Please avoid repeated freezing and thawing. You can refer to our peptide storage guidelines for details.