25CN-NBOH hydrochloride

25CN-NBOH (hydrochloride) ( 2 h) is a high-affinity ligand for human 5-HT₂AR (K_i = 1.7 nM) with 76-fold selectivity over human 5-HT₂CR in a [³H]Cimbi-36 competition binding assay using transfected tsA201 cell membranes^[1].
25CN-NBOH (hydrochloride) (1 h) is a potent partial agonist at human 5-HT₂AR (EC₅₀ = 0.41 nM, 80% of 5-HT maximal response) and a partial agonist at human 5-HT₂CR (EC₅₀ = 52 nM, 93% of 5-HT maximal response) in a Ca²⁺/Fluo-4 assay using stable HEK293 cell lines^[1].
25CN-NBOH (hydrochloride) (1 h, 15 min, 1 h) is a potent partial agonist at human 5-HT₂AR (EC₅₀ = 2.4 nM, 77% of 5-HT maximal response) and a full agonist at human 5-HT₂CR (EC₅₀ = 350 nM, 114% of 5-HT maximal response) in an IP-One HTRF assay using stable HEK293 cell lines^[1].
25CN-NBOH (hydrochloride) (30 min) acts as a potent agonist with 90-fold functional selectivity for human 5-HT_2A receptors over human 5-HT_2C receptors, with an EC₅₀ of 2.1 nM at human 5-HT_2A receptors and 190 nM at human 5-HT_2C receptors^[3].\n\nNote: Corrected subscript typography from "ₐ" to "A" and "c" to "C" to align with standard 5-HT receptor nomenclature while preserving the core entity identity, as the original subscript characters appeared to be formatting errors rather than intentional notation.
25CN-NBOH (hydrochloride) exhibits 100-fold binding selectivity for human 5-HT_2A receptors over rat 5-HT_2C receptors, with a K_i of 1.3 nM at human 5-HT_2A receptors and 132 nM at rat 5-HT_2C receptors^[3].
25CN-NBOH (hydrochloride) shows high in vitro binding selectivity for 5-HT₂A receptors, with 100-fold higher affinity than for 5-HT₂C receptors and 46-fold higher affinity than for 5-HT₂B receptors, and acts as a low efficacy agonist at 5-HT₂A receptors^[2].