2C-B-FLY hydrochloride

2C-B-FLY hydrochloride inhibits NET, DAT, and SERT in HEK 293 cells with IC₅₀ values of 94, 187, and 73 μM, respectively^[1].
2C-B-FLY hydrochloride binds with high affinity to rat TAAR₁ (K_i = 0.03 μM) and human 5-HT_2A (K_i = 0.01 μM), and exhibits lower-affinity binding to monoamine transporters and other receptors in HEK 293 membrane preparations^[1].
2C-B-FLY (100 μM; 4 h) hydrochloride does not produce cytotoxicity in HEK 293^[1].
2C-B-FLY hydrochloride (5-500 μM; 120 min) is metabolized by CYP2D6 in human liver microsomes (HLM) and recombinant monoamine oxidase and competitively inhibits MAO-A activity^[2].
2C-B-FLY (1 pM-100 µM; 2 h) hydrochloride acts as a 5-HT_2A receptor agonist in stably expressing HEK 293 T cells, inducing β-arrestin 2 recruitment with an EC₅₀ of 8.11 nM and an E_max of 81.8% relative to the reference agonist^[4].
2C-B-FLY (10 pM-10 μM; 80 s) hydrochloride is a highly potent agonist of Gαq-mediated calcium mobilization in HEK293 cells expressing human 5-HT_2A receptors, with an E_MAX of 99.05% and a pEC₅₀ of -7.27^[5].
2C-B-FLY hydrochloride (2-1250 μM; 20 min) inhibits human recombinant MAO-A in a cell-free system with an IC₅₀ of 19 µM, but is inactive against human recombinant MAO-B^[6].
2C-B-FLY hydrochloride (25 µM; 2 h) has no receptor-activating effect in HEK293T cells expressing the human μ opioid receptor (MOR)^[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.