4-Hydrazinobenzoic acid
Based on 1 Customer Validation
4-Hydrazinobenzoic acid (p-Hydrazinobenzoic acid) is a DNA-damaging agent. 4-Hydrazinobenzoic acid induces copper-dependent oxidative damage, accompanied by strand breaks and base modifications; autoxidation generates hydrogen peroxide and cuprous ion-derived ROS. 4-Hydrazinobenzoic acid is cytotoxic to cancer cells and normal cells. 4-Hydrazinobenzoic acid can be used in cancer research.
For research use only. We do not sell to patients.
- Purity : 99.68%
- CAS No.: 619-67-0
- Formula: C7H8N2O2
- Molecular Weight:152.15
-
Storage:
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Protocols
- Purity & Documentation
- References
-
Help & FAQs
-
HY-W017382R
4-Hydrazinobenzoic acid (Standard)Reference Standard -
HY-41677
4-Cyanophenylhydrazine hydrochlorideBiochemical Reagent -
HY-W001874
2-CyanoethylhydrazineDrug Intermediate -
HY-W115752
Hydrazine sulfate (99%)PEPCK Inhibitor -
HY-W094747A
BenzalazineAnti-Ulcerative Colitis Agent -
HY-I0777
1,2-DiacetylhydrazineDrug Impurity -
HY-W012890
PentanehydrazideBiochemical Assay Reagents -
HY-I0777R
1,2-Diacetylhydrazine (Standard)
All DNA/RNA Synthesis Isoforms
More
Biological Activity
Description
Cellular Effect
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| HCT-116 | IC50 |
34.6 μM
|
Cytotoxicity against human HCT-116 cells assessed as viability reduction by MTT assay.
Cytotoxicity against human HCT-116 cells assessed as viability reduction by MTT assay.
|
32702510 |
| MCF7 | IC50 |
34.6 μM
|
Cytotoxicity against human MCF-7 cells assessed as viability reduction by MTT assay.
Cytotoxicity against human MCF-7 cells assessed as viability reduction by MTT assay.
|
32702510 |
In Vitro
4-Hydrazinobenzoic acid (concentration-dependent) combined with Cu (II) increases the formation of 8-oxodG in calf thymus DNA, whereas catalase prevents this increase[2].
4-Hydrazinobenzoic acid (20 µM; 60 min) induces Cu (II)-dependent DNA damage in 32P-labeled human p53 and p16 tumor suppressor gene fragments, and 20 µM gives rise to H2O2/Cu (I)-mediated T/C piperidine-labile sites, whereas at high concentrations combined with catalase it causes non-oxidative A/G/C sites[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 619-67-0
-
Appearance Solid
-
Molecular Weight 152.15
-
Formula C7H8N2O2
-
Color Yellow to brown
-
SMILES
O=C(O)C1=CC=C(NN)C=C1
-
Synonyms
p-Hydrazinobenzoic acid
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (657.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (278 KB)
-
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 6.5725 mL | 32.8623 mL | 65.7246 mL | 164.3115 mL |
| 5 mM | 1.3145 mL | 6.5725 mL | 13.1449 mL | 32.8623 mL | |
| 10 mM | 0.6572 mL | 3.2862 mL | 6.5725 mL | 16.4312 mL | |
| 15 mM | 0.4382 mL | 2.1908 mL | 4.3816 mL | 10.9541 mL | |
| 20 mM | 0.3286 mL | 1.6431 mL | 3.2862 mL | 8.2156 mL | |
| 25 mM | 0.2629 mL | 1.3145 mL | 2.6290 mL | 6.5725 mL | |
| 30 mM | 0.2191 mL | 1.0954 mL | 2.1908 mL | 5.4771 mL | |
| 40 mM | 0.1643 mL | 0.8216 mL | 1.6431 mL | 4.1078 mL | |
| 50 mM | 0.1314 mL | 0.6572 mL | 1.3145 mL | 3.2862 mL | |
| 60 mM | 0.1095 mL | 0.5477 mL | 1.0954 mL | 2.7385 mL | |
| 80 mM | 0.0822 mL | 0.4108 mL | 0.8216 mL | 2.0539 mL | |
| 100 mM | 0.0657 mL | 0.3286 mL | 0.6572 mL | 1.6431 mL |
4-Hydrazinobenzoic acid Related Classifications
HY-W017382 Related Classifications
Powered by Bioz