4L2-P13D
Based on 1 Customer Validation
4L2-P13D is an IL-11 antagonist with an IC50 of 2.8 μM. 4L2-P13D has a KD of 11 nM for hIL-11. 4L2-P13D exhibits antifibrotic and renoprotective activities. 4L2-P13D can be used in the research of renal fibrosis.
For research use only. We do not sell to patients.
- Purity : 98.98%
- Formula: C89H123N19O23S
- Molecular Weight:1859.11
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
4L2-P13D (0.5-5 μM; 72 h) reduces the expression of profibrotic factors (FN1, COL1A1) in TGF-β1-induced HK-2 cells in a dose-dependent manner[1].
4L2-P13D (0-50 μM; 24 h) exhibits no cytotoxicity against HK-2 cells even at concentrations as high as 50 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:TGF-β1-induced human proximal tubular epithelial (HK-2) cells
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Concentration:0.5, 2 and 5 μM
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Incubation Time:72 h
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Result:Dose-dependently reduced fibronectin (FN1) and type I collagen (COL1A1) expression.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6J (male, 6-8 weeks old, UUO model)[1]
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Dosage:0.5 mg/kg; 2 mg/kg
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Administration:s.c.; daily; 7 days
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Result:Significantly reduced expression levels of fibrosis markers FN1 and α-smooth muscle α-SMA.
Remarkably reduced proinflammatory markers (TNF-α, TGF-β1, IL-6, IL-1β) and kidney injury markers (Kim1, Ngal).
Reduced blood urea nitrogen (BUN) and creatinine levels.
Significantly improved collagen deposition, with the high-dose group showing superior efficacy compared to the low-dose group.
Chemical Information
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Appearance Solid
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Molecular Weight 1859.11
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Formula C89H123N19O23S
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Color White to off-white
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Sequence
Ac-Tyr-Leu-Ile-Gln-His-Tyr-Tyr-Thr-Phe-Ile-Ala-Leu-Asp-Cys-Gly-NH2 (sulfide bridge: Tyr1-Cys14)
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Sequence Shortening
Ac-YLIQHYYTFIALDCG-NH2 (sulfide bridge: Tyr1-Cys14)
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (53.79 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (1.34 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (1.34 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (288 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 0.5379 mL | 2.6895 mL | 5.3789 mL | 13.4473 mL |
| 5 mM | 0.1076 mL | 0.5379 mL | 1.0758 mL | 2.6895 mL | |
| 10 mM | 0.0538 mL | 0.2689 mL | 0.5379 mL | 1.3447 mL | |
| 15 mM | 0.0359 mL | 0.1793 mL | 0.3586 mL | 0.8965 mL | |
| 20 mM | 0.0269 mL | 0.1345 mL | 0.2689 mL | 0.6724 mL | |
| 25 mM | 0.0215 mL | 0.1076 mL | 0.2152 mL | 0.5379 mL | |
| 30 mM | 0.0179 mL | 0.0896 mL | 0.1793 mL | 0.4482 mL | |
| 40 mM | 0.0134 mL | 0.0672 mL | 0.1345 mL | 0.3362 mL | |
| 50 mM | 0.0108 mL | 0.0538 mL | 0.1076 mL | 0.2689 mL |
4L2-P13D Related Classifications
HY-P11623 Related Classifications
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