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Cat. No.: HY-W746773

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5-Hydroxy Indoleacetylglycine

5-Hydroxy Indoleacetylglycine is an indole derivative metabolite derived from tryptophan metabolism, and its production depends on the gut microbiota and is associated with tryptophan metabolism and intestinal Clostridium colonization. 5-Hydroxy Indoleacetylglycine can be used for research on gut microbial metabolism and tryptophan metabolic pathways.

For research use only. We do not sell to patients.

CAS No.: 16606-62-5
Formula: C₁₂H₁₂N₂O₄
Molecular Weight:248.24

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

5-Hydroxy Indoleacetylglycine Chemical Structure

CAS No.: 16606-62-5

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Purity & Documentation
References
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Biological Activity

Description

In Vitro

5-Hydroxyindoleacetylglycine (50 mg sample in 400 µL methanol:water (4:1, v/v); 60 days (ensiling)) is upregulated in whole-plant mulberry silage in the fresh organic acid treatment group compared with the fresh Lactiplantibacillus plantarum inoculation group, and both differential metabolites are enriched in the tryptophan metabolism pathway^[1].
5-Hydroxy-indoleacetylglycine is significantly elevated in rat feces in the comparison between the C and WP groups^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

5-Hydroxy-indoleacetylglycine is a fecal indole derivative metabolite whose production depends on gut microflora and correlates with Clostridia_UCG-014 and Clostridium_sensu_stricto_1, with changes likely attributable to tryptophan metabolism^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Related Protocols :

Research Protocol for Microbiome Analysis

Chemical Information

CAS No. 16606-62-5
Molecular Weight 248.24
Formula C₁₂H₁₂N₂O₄
SMILES

O=C(O)CNC(=O)CC1=CNC=2C=CC(O)=CC21
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Di F, et al. Effects of wilting and additives on fermentation characteristics, microbial composition, metabolome, and ruminal degradation properties of mulberry silage. BMC microbiology. 2026 Jan 10;26(1):139.

[2]. Zhuang K, et al. Fecal metabonomics combined with 16S rDNA sequencing to analyze the changes of gut microbiota in rats fed with different protein source diets. European journal of nutrition. 2023 Sep;62(6):2687-2703.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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