5-Methyl-DL-tryptophan
Based on 1 Customer Validation
5-Methyl-DL-tryptophan is an orally active amino acid derivative with anti-inflammatory signaling effects. 5-Methyl-DL-tryptophan inhibits TLR4/MyD88 signaling and NF-κB activation/phosphorylation, reduces IL-6/IL-1β/TNF-α, and decreases the p-p65/p65 ratio. 5-Methyl-DL-tryptophan inhibits anthranilate synthase (Ki = 13 μM). 5-Methyl-DL-tryptophan inhibits bacterial growth. 5-Methyl-DL-tryptophan can be used in research on ulcerative colitis.
For research use only. We do not sell to patients.
- Purity : 99.94%
- CAS No.: 951-55-3
- Formula: C12H14N2O2
- Molecular Weight:218.26
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
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TLR4 |
NF-κB |
MyD88 |
IL-6 |
IL-1β |
TNF-α |
p-p65/p65 |
anthranilate synthase 13 μM (Ki) |
In Vitro
5-Methyl-DL-tryptophan (5-MT) inhibits wild-type E. coli K-12 anthranilate synthase with a Ki of 13 µM[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/N (SPF, male, 6-week-old)[1]
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Dosage:30 mg/kg
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Administration:p.o. (orally)
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Result:Reduced DSS-induced body weight loss and decreased DAI score compared with the DSS model.
Lowered serum LPS level compared with the DSS model.
Decreased protein levels of IL-6, IL-1β, and TNF-α in colon and serum compared with the DSS model.
Decreased colonic mRNA expression of IL-6, IL-1β, TNF-α, TLR4, MyD88, and NF-κB compared with the DSS model.
Attenuated colonic tissue damage and colon length shortening; HAI score and colon length determination indicated reduced severity of UC compared with the DSS model.
Improved DSS-induced gut microbiota disorder; α-diversity analysis suggested a repairing effect on DSS-induced dysbiosis.
Altered the relative abundance of Alistipes, Ralstonia, and Akkermansia.
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Animal Model:albino[3]
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Dosage:50 mg/kg
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Administration:i.p.; once
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Result:At 50 mg/kg i.p., 5-methyl-DL-tryptophan produced % i values of 3 and 3 for inhibition of Chloroquine-induced pigment clumping.
Chemical Information
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CAS No. 951-55-3
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Appearance Solid
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Molecular Weight 218.26
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Formula C12H14N2O2
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Color White to off-white
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
1 M HCl : ≥ 100 mg/mL (458.17 mM)
DMSO : 66.67 mg/mL (305.46 mM; ultrasonic and adjust pH to 2 with 1 M HCl; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (22.91 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (22.91 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (294 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO / 1 M HCl | 1 mM | 4.5817 mL | 22.9085 mL | 45.8169 mL | 114.5423 mL |
| 5 mM | 0.9163 mL | 4.5817 mL | 9.1634 mL | 22.9085 mL | |
| 10 mM | 0.4582 mL | 2.2908 mL | 4.5817 mL | 11.4542 mL | |
| 15 mM | 0.3054 mL | 1.5272 mL | 3.0545 mL | 7.6362 mL | |
| 20 mM | 0.2291 mL | 1.1454 mL | 2.2908 mL | 5.7271 mL | |
| 25 mM | 0.1833 mL | 0.9163 mL | 1.8327 mL | 4.5817 mL | |
| 30 mM | 0.1527 mL | 0.7636 mL | 1.5272 mL | 3.8181 mL | |
| 40 mM | 0.1145 mL | 0.5727 mL | 1.1454 mL | 2.8636 mL | |
| 50 mM | 0.0916 mL | 0.4582 mL | 0.9163 mL | 2.2908 mL | |
| 60 mM | 0.0764 mL | 0.3818 mL | 0.7636 mL | 1.9090 mL | |
| 80 mM | 0.0573 mL | 0.2864 mL | 0.5727 mL | 1.4318 mL | |
| 100 mM | 0.0458 mL | 0.2291 mL | 0.4582 mL | 1.1454 mL |
5-Methyl-DL-tryptophan Related Classifications
HY-33228 Related Classifications
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Keywords
- 5-Methyl-DL-tryptophan
- 951-55-3
- Amino Acid Derivatives
- Toll-like Receptor (TLR)
- MyD88
- NF-κB
- Interleukin Related
- TNF Receptor
- Bacterial
- 7-DMATSNeo
- Akkermansia
- C7-prenylation
- NF-κB activation
- TLR4/MyD88 signaling
- anthranilate synthetase
- inflammatory signaling
- tight-junction
- tryptophan pyrrolase
- ulcerative colitis
- Inhibitor
- inhibitor
- inhibit