Cat. No.: HY-N21597

8-Acetonyldihydronitidine

8‑Acetonyldihydronitidine is a naturally occurring benzophenanthridine alkaloid with antibacterial, antifungal, and anti-proliferative activities against colorectal cancer cells. 8‑Acetonyldihydronitidine inhibits Staphylococcus aureus and exerts a potent antifungal effect against Cladosporium cladosporioides. 8‑Acetonyldihydronitidine activates the p53 signaling pathway, upregulates the expression of target genes including p21, BAX, and FAS, downregulates cyclin A and cyclin B, and induces cell cycle arrest and apoptosis, thereby suppressing the proliferation of colorectal cancer cells. 8‑Acetonyldihydronitidine is used in research related to colorectal cancer, fungal infections, and bacterial infections.

For research use only. We do not sell to patients.

CAS No.: 80330-39-8
Formula: C₂₄H₂₃NO₅
Molecular Weight:405.45

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

8-Acetonyldihydronitidine Chemical Structure

CAS No.: 80330-39-8

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

^[1]

CDK1/cyclin A

CDK2(C118L/A144C-Cyclin B)

Caspase 3

Bax

All CDK Isoforms :

All Bcl-2 Family Isoforms :

All Caspase Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	IC50	5.38 μM	Antiproliferative activity against human HCT116 colorectal cancer cells assessed as reduction in cell viability incubated for 48 h by MTT assay. Antiproliferative activity against human HCT116 colorectal cancer cells assessed as reduction in cell viability incubated for 48 h by MTT assay.	j.ejphar.2019.03.042
LoVo	IC50	5.81 μM	Antiproliferative activity against human LOVO colorectal cancer cells assessed as reduction in cell viability incubated for 48 h by MTT assay. Antiproliferative activity against human LOVO colorectal cancer cells assessed as reduction in cell viability incubated for 48 h by MTT assay.	j.ejphar.2019.03.042
HCT-15	IC50	12.91 μM	Antiproliferative activity against human HCT15 colorectal cancer cells assessed as reduction in cell viability incubated for 48 h by MTT assay. Antiproliferative activity against human HCT15 colorectal cancer cells assessed as reduction in cell viability incubated for 48 h by MTT assay.	j.ejphar.2019.03.042
SW480	IC50	9.33 μM	Antiproliferative activity against human SW480 colorectal cancer cells assessed as reduction in cell viability incubated for 48 h by MTT assay. Antiproliferative activity against human SW480 colorectal cancer cells assessed as reduction in cell viability incubated for 48 h by MTT assay.	j.ejphar.2019.03.042

In Vitro

Anti-cancerCell Cycle RegulationApoptosisBacterial InfectionFungal Infection

Anti-cancer

Cell Cycle Regulation

8-Acetonyldihydronitidine (8-AHN) (0-80 µM; 48 h) effectively inhibits the proliferation of HCT116, LOVO, HCT15, and SW480 human colorectal cancer cells, with the lowest IC₅₀ of 5.38 µM observed in HCT116 cells^[1].
8-Acetonyl dihydronitidine (5 µM; 0-48 h) induces G2/M phase cell cycle arrest in synchronized HCT116 human colorectal cancer cells in a time-dependent manner^[1].
8-Acetonyl dihydronitidine (0-8 µM; 24 h) downregulates cyclin A and cyclin B at both the protein and mRNA levels in HCT116 and LOVO human colorectal cancer cells^[1].

Anti-cancer

Apoptosis

8-Acetonyl dihydronitidine (2-10 µM; 0-48 h) induces apoptosis in HCT116 human colorectal cancer cells in a time- and dose-dependent manner, and the level of cleaved caspase-3 increases after treatment at a concentration of 8 µM for 24 h^[1].

Anti-cancer

Cell Cycle Regulation

Apoptosis

8-Acetonyldihydronitidine enhances the expression and transcriptional activity of p53 in HCT116 human colorectal cancer cells, and upregulates the p53 target genes p21, BAX and FAS after 24 h of treatment^[1].

Bacterial Infection

8-Acetonyl dihydronitidine (compound 1) inhibits the growth of Staphylococcus aureus, with an MIC of 1.56 μg/mL and an MBC of 25 μg/mL^[2].

Fungal Infection

8-Acetonydihydronitidine exhibits potent antifungal activity against Cladosporium cladosporioides (100 mm² inhibition zone at 2 mg), and inhibits more than 90% of conidial germination of Colletotrichum gloeosporioides after 6 h of treatment at a concentration of 200 μg/mL^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

HCT116, HCT15, LOVO, SW480 human colorectal cancer cell lines
Concentration:

0, 0.25, 0.5, 1, 2.5, 5, 10, 20, 40, 80 µM
Incubation Time:

48 h
Result:

Suppressed proliferation in all four cell lines in a concentration-dependent manner.
Exhibited IC₅₀ values of 5.38 µM for HCT116 cells, 5.81 µM for LOVO cells, 12.91 µM for HCT15 cells, and 9.33 µM for SW480 cells.
Showed the highest sensitivity in HCT116 and LOVO cells.

Cell Cycle Analysis^[1]

Cell Line:

synchronized HCT116 human colorectal cancer cells
Concentration:

5 µM
Incubation Time:

0 h, 24 h, 48 h
Result:

Caused a time-dependent increase in the proportion of cells in the G2/M phase.
Increased G2/M phase proportion from 29.2% at 0 h to 53.1% at 24 h and 57.9% at 48 h.

Western Blot Analysis^[1]

Cell Line:

HCT116 and LOVO human colorectal cancer cells
Concentration:

0, 2, 4, 6, 8, 10 µM
Incubation Time:

24 h
Result:

Decreased the protein levels of cyclin A and cyclin B in both HCT116 and LOVO cells in a dose-dependent manner.
Significantly increased the protein level of cleaved caspase-3 at the 8 µM dose along with other cleaved apoptotic markers in a dose-dependent manner after 24 h.
Significantly increased protein level of p53 transcriptional targets p21, BAX, and FAS upon treatment.

Real Time qPCR^[1]

Cell Line:

HCT116 and LOVO human colorectal cancer cells
Concentration:

0, 4, 8 µM
Incubation Time:

24 h
Result:

Reduced mRNA expression of both cyclin B and cyclin A within 24 h of treatment in a dose-dependent manner.
Significantly increased mRNA level of p53 transcriptional targets p21, BAX, and FAS upon treatment.

Apoptosis Analysis^[1]

Cell Line:

HCT116 human colorectal cancer cells
Concentration:

5 µM
Incubation Time:

0 h, 24 h, 48 h
Result:

Increased the combined percentage of early and late apoptotic cells from 2.35% at 0 h to 21.49% at 24 h and 31.57% at 48 h at 5 µM.

In Vivo

Anti-cancerApoptosisCell Cycle Regulation

Anti-cancer

Apoptosis

Cell Cycle Regulation

8-Acetonyldihydronitidine (8-AHN) (10 mg/kg; i.p.; every other day; 2 weeks) potently suppresses HCT116 colorectal xenograft tumor growth in mice via proliferation suppression and apoptosis induction, without notable systemic toxicity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

BALB/c nude mice (male; 5 weeks old; 15-18 g; HCT116 human colorectal cancer cell subcutaneous xenograft)^[1]
Dosage:

10 mg/kg
Administration:

i.p.; every other day; 2 weeks
Result:

Significantly inhibited the growth of HCT116 xenograft tumors.
Reduced average excised tumor weight to 300 mg.
Decreased tumor volume relative to the vehicle control group.
Did not cause significant body weight loss in treated animals.
Increased expression of BAX and p53 in tumor sections.
Decreased expression of cyclin B and PCNA in tumor sections.
Showed no major organ-related toxicities in lung and liver.

Related Protocols :

Chemical Information

CAS No. 80330-39-8
Molecular Weight 405.45
Formula C₂₄H₂₃NO₅
SMILES

O=C(C)CC1C2=CC(OC)=C(OC)C=C2C=3C=CC4=CC=5OCOC5C=C4C3N1C
Structure Classification
- Alkaloids
- Quinoline Alkaloids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Zhou J W, et al. 8‑Acetonyldihydronitidine inhibits the proliferation of human colorectal cancer cells via activation of p53. European Journal of Pharmacology, 2019, 854: 256‑264.

[2]. Nissanka AP, et al. Antimicrobial alkaloids from Zanthoxylum tetraspermum and caudatum. Phytochemistry. 2001 Apr;56(8):857-61.

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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8-Acetonyldihydronitidine

All CDK Isoforms

All Bcl-2 Family Isoforms

All Caspase Isoforms