Gln-AMS TFA
Based on 3 publication(s) in Google Scholar
Gln-AMS TFA is a potent inhibitor of Aminoacyl-tRNA Synthetase. Gln-AMS TFA blocks the lactylation modification of downstream targets through competitive binding to AARS1, thereby regulating apoptosis, ferroptosis, and the transcriptional processes of related genes. Gln-AMS TFA can be used in research on breast cancer, diabetic nephropathy, and sepsis-associated encephalopathy.
For research use only. We do not sell to patients.
- Purity : 99.85%
- Formula: C17H23F3N8O10S
- Molecular Weight:588.47
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Storage:
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Gln-AMS TFA
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Biological Activity
Description
IC50 & Target
[1]|
AARS1 |
GlnRS |
In Vitro
Gln-AMS (1 μM; 72 h) TFA decreased ELOVL5 protein and mRNA expression in HGECs and HK-2 cells[2].
Gln-AMS (10 μM; 2 h) TFA inhibits AARS1-mediated global protein lactylation and ATRIP lactylation in primary hippocampal neurons[3].
Gln-AMS (10 μM; 12 h) TFA inhibits Lactic acid (HY-B2227)-induced global protein lactylation and p-ATR, p-CHK1 expression in primary hippocampal neurons[3].
Gln-AMS (10 μM; 12 h) TFA inhibits p-ATR and p-CHK1 expression in HT22 cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MDA-MB-468 and T-47D cells
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Concentration:10 μM
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Incubation Time:24 h
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Result:Decreased global Kla, H4K79la, and H4K91la levels.
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Cell Line:HT22
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Concentration:as indicated (Gln-AMS); 1 μg/mL (LPS); varying concentrations (lactate)
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Incubation Time:12 h
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Result:Modulated p-ATR (1989T) and p-CHK1 levels in LPS and lactate-treated HT22 cells.
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Cell Line:Primary hippocampal neurons
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Concentration:10 μM
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Incubation Time:2, 12 h
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Result:Inhibited overall protein lactylation and ATRIP lactylation in the cytoplasm. The product also decreased the expression of p-ATR and p-CHK1 induced by lactate.
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Cell Line:HT22
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Concentration:10 μM
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Incubation Time:12 h
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Result:Suppressed the expression of p-ATR and p-CHK1.
Chemical Information
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Appearance Solid
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Molecular Weight 588.47
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Formula C17H23F3N8O10S
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Color White to off-white
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SMILES
NC1=C2N=CN([C@@H]3O[C@H](COS(=O)(NC([C@@H](N)CCC(N)=O)=O)=O)[C@@H](O)[C@H]3O)C2=NC=N1.OC(C(F)(F)F)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Publications (3)
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Journal Impact Factor
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Most Recent
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Adv Sci (Weinh)
Lactylated Apolipoprotein C-II Induces Immunotherapy Resistance by Promoting Extracellular Lipolysis. [Abstract]2024 Oct;11(38):e2406333. PMID: 38981044 -
Cell Death Differ
AARS1-mediated lactylation of H3K18 and STAT1 promotes ferroptosis in diabetic nephropathy. [Abstract]2025 Sep 23. PMID: 40987895 -
Brain Behav Immun
Targeting AARS1-dependent lactylation improves neuronal process plasticity and mitigates cognitive deficits in sepsis-associated encephalopathy. [Abstract]2026 Jul:135:106493. PMID: 41713664
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (169.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.25 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.25 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (283 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Liu J, et al. H4K79 and H4K91 histone lactylation, newly identified lactylation sites enriched in breast cancer. Journal of experimental & clinical cancer research : CR. 2025 Aug 23;44(1):252. [Content Brief]
[2]. Hong J, et al. AARS1-mediated lactylation of H3K18 and STAT1 promotes ferroptosis in diabetic nephropathy. Cell death and differentiation. 2026 Mar;33(3):589-604. [Content Brief]
[3]. Luo S, et al. Targeting AARS1-dependent lactylation improves neuronal process plasticity and mitigates cognitive deficits in sepsis-associated encephalopathy. Brain, behavior, and immunity. 2026 Jul;135:106493. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.6993 mL | 8.4966 mL | 16.9932 mL | 42.4831 mL |
| 5 mM | 0.3399 mL | 1.6993 mL | 3.3986 mL | 8.4966 mL | |
| 10 mM | 0.1699 mL | 0.8497 mL | 1.6993 mL | 4.2483 mL | |
| 15 mM | 0.1133 mL | 0.5664 mL | 1.1329 mL | 2.8322 mL | |
| 20 mM | 0.0850 mL | 0.4248 mL | 0.8497 mL | 2.1242 mL | |
| 25 mM | 0.0680 mL | 0.3399 mL | 0.6797 mL | 1.6993 mL | |
| 30 mM | 0.0566 mL | 0.2832 mL | 0.5664 mL | 1.4161 mL | |
| 40 mM | 0.0425 mL | 0.2124 mL | 0.4248 mL | 1.0621 mL | |
| 50 mM | 0.0340 mL | 0.1699 mL | 0.3399 mL | 0.8497 mL | |
| 60 mM | 0.0283 mL | 0.1416 mL | 0.2832 mL | 0.7081 mL | |
| 80 mM | 0.0212 mL | 0.1062 mL | 0.2124 mL | 0.5310 mL | |
| 100 mM | 0.0170 mL | 0.0850 mL | 0.1699 mL | 0.4248 mL |
Gln-AMS TFA Related Classifications
HY-112861A Related Classifications
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