Zinquin
Based on 1 Customer Validation
Zinquin is a fluorescent zinc ion (Zn2+) indicator. Zinquin can form a fluorescent complex with Zn2+, which is used to study zinc distribution in cells and tissues, zinc-related physiological processes, and changes in intracellular zinc homeostasis (Ex/Em = 368/490 nm).
For research use only. We do not sell to patients.
- Purity : 99.77%
- CAS No.: 151606-29-0
- Formula: C19H18N2O5S
- Molecular Weight:386.42
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
1. Stock solution preparation
1.1 Solvent: anhydrous MDSO.
1.2 Recommended concentration: approximately 25 mM.
2. Working solution preparation
2.1 Diluent: physiological saline (124 mM NaCl, 2.5 mM KCl, 2 mM CaCl2, 2 mM MgCl2, 23 mM NaHCO3, 10 mM glucose, bubbled with 95/5% O2/CO2 gas mixture).
2.2 Working concentration: 25 μM Zinquin.
2.3 Note: Adjust the working solution concentration as needed; prepare fresh before use.
3. Staining procedure
3.1 Sample type: rat hippocampal or neocortical slices (400 μm) prepared using a McIlwain tissue chopper; before staining, keep the slices in physiological saline in an interface holding chamber at room temperature.
3.2 Incubation conditions: continuously perfuse the slices with physiological saline containing 25 μM Zinquin at room temperature.
4. Controls
4.1 In liposome experiments, the membrane-permeable Zn2+ chelator TPEN quenches Zinquin fluorescence; no signal is generated after pre-incubation with TPEN.
5. Detection and analysis
5.1 Instrument type: fluorescence microscope.
5.2 Wavelength settings: excitation wavelength 360 nm; 400 nm dichroic mirror; emission filter 510/80 nm.
5.3 Result analysis: Zinquin fluorescence is expected to be visible in the dentate gyrus hilus and mossy fiber projection areas, with less labeling of cell bodies; individual mossy fiber terminals are distinguishable at high magnification.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Emission (Em)
490
Chemical Information
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CAS No. 151606-29-0
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Appearance Solid
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Molecular Weight 386.42
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Formula C19H18N2O5S
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Color White to off-white
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SMILES
O=C(O)COC1=CC(NS(=O)(C2=CC=C(C)C=C2)=O)=C3N=C(C)C=CC3=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
In Vitro:
DMSO : 50 mg/mL (129.39 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (279 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.5879 mL | 12.9393 mL | 25.8786 mL | 64.6964 mL |
| 5 mM | 0.5176 mL | 2.5879 mL | 5.1757 mL | 12.9393 mL | |
| 10 mM | 0.2588 mL | 1.2939 mL | 2.5879 mL | 6.4696 mL | |
| 15 mM | 0.1725 mL | 0.8626 mL | 1.7252 mL | 4.3131 mL | |
| 20 mM | 0.1294 mL | 0.6470 mL | 1.2939 mL | 3.2348 mL | |
| 25 mM | 0.1035 mL | 0.5176 mL | 1.0351 mL | 2.5879 mL | |
| 30 mM | 0.0863 mL | 0.4313 mL | 0.8626 mL | 2.1565 mL | |
| 40 mM | 0.0647 mL | 0.3235 mL | 0.6470 mL | 1.6174 mL | |
| 50 mM | 0.0518 mL | 0.2588 mL | 0.5176 mL | 1.2939 mL | |
| 60 mM | 0.0431 mL | 0.2157 mL | 0.4313 mL | 1.0783 mL | |
| 80 mM | 0.0323 mL | 0.1617 mL | 0.3235 mL | 0.8087 mL | |
| 100 mM | 0.0259 mL | 0.1294 mL | 0.2588 mL | 0.6470 mL |
Zinquin Related Classifications
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