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Cat. No.: HY-159700

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AA3-DLin

Name: AA3-DLin
Brand: MedChemExpress
SKU: HY-159700
Price: 125.00 USD
Availability: InStock
Rating: 5 (1 reviews)

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AA3-DLin is a biodegradable ionizable cationic lipid (pK_a = 5.8). AA3-DLin is used to combine mRNA with lipid nanoparticles (LNPs) for delivery, thereby achieving endosomal escape and cytoplasmic release. AA3-DLin can be used in research on type 2 diabetes, steroid-resistant asthma, lung cancer, ischemic stroke, breast cancer, melanoma, and COVID-19.

For research use only. We do not sell to patients.

Purity : 99.0%
CAS No.: 3027565-91-6
Formula: C₄₄H₇₈N₂O₄
Molecular Weight:699.10

Storage:

Solution, -20°C, 2 years

AA3-DLin Chemical Structure

CAS No.: 3027565-91-6

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

In Vitro

AA3-DLin LNPs (0-64 µg/mL; 24 h) exhibit low cytotoxicity in differentiated C2C12 myotubes^[1].
The AA3-DLin-based LNP formulation (4 h) demonstrates efficient cellular uptake across LLC, B16F10, and 4T1 cancer cell lines, and this uptake is not altered by nebulization^[3].
The AA3-DLin-based LNP formulation (4-48 h) effectively mediates gene transfection and PD-L1 silencing in both murine and human lung cancer cell lines, and this efficacy is preserved after nebulization^[3].
The AA3-DLin-based LNP formulation (8-72 h) effectively delivers mscFv to LLC, B16F10, and 4T1 cells, leading to intracellular expression of anti-DDR1 scFv and its sustained secretion^[3].
AA3-Dlin LNPs (0.5-4 h) are efficiently internalized by HEK 293, HeLa, 4T1, and B16F10 cells in a time-dependent manner^[5].
AA3-Dlin LNPs (0.5 μg/mL; 4 h) facilitate endosomal escape to release mRNA into the cytosol of HEK 293, HeLa, 4T1, and B16F10 cells^[5].
The optimized A12 AA3-DLin LNP formulation (0.1 μg; 24 h) demonstrates superior in vitro mRNA transfection efficacy in Hek 293 cells compared to MC3 LNPs, lipofectamine 3000, and ALC-0315 LNPs, with no observed cytotoxicity^[6].
AA3-DLin LNPs (72 h) demonstrate excellent mRNA delivery efficiency and can be used for mRNA vaccine development and gene therapy research^[8].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Viral InfectionInnate ImmunityOthers

Viral Infection

AA3-DLin (2-10 μg spike mRNA-loaded AA3-DLin vaccines; i.m.; prime at day 0 and boost at day 14) COVID-19 vaccines induce strong spike-specific antibody responses in a dose-dependent manner^[6].

Innate Immunity

AA3-DLin LNPs are an excellent mRNA delivery platform that successfully induces strong immunogenicity in BALB/c mice, demonstrating potential for vaccine development and gene therapy^[8].

Others

AA3-DLin (10 μg mRNA (0.5 mg/kg); i.m.) LNPs efficiently deliver luciferase mRNA in vivo, generating strong luciferase expression of 2.35 × 10⁸ total flux (p/s) at 6 h post-injection^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Related Protocols :

Chemical Information

CAS No. 3027565-91-6
Appearance Liquid
Molecular Weight 699.10
Formula C₄₄H₇₈N₂O₄
Color Light yellow to yellow
SMILES

CCCCC/C=C\C/C=C\CCCCCCCC(OCCN1CCN(CC1)CCOC(CCCCCCC/C=C\C/C=C\CCCCC)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Solution, -20°C, 2 years

Solvent & Solubility

In Vitro:

Ethanol : 50 mg/mL (71.52 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (270 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. El-Araby RE, et al. Adiponectin mRNA Conjugated with Lipid Nanoparticles Specifically Targets the Pathogenesis of Type 2 Diabetes. Aging and disease. 2024 May 15;16(2):1059-1079.

[2]. Huang J, et al. Budesonide-incorporated inhalable lipid nanoparticles for antiTSLP nanobody mRNA delivery to treat steroid-resistant asthma. Nature communications. 2025 Jul 01;16(1):6013.

[3]. Hu B, et al. Modulating tumor collagen fiber alignment for enhanced lung cancer immunotherapy via inhaled RNA. Nature communications. 2025 Aug 30;16(1):8120. [Content Brief]

[4]. Gao M, et al. Targeted mRNA Nanoparticles Ameliorate Blood-Brain Barrier Disruption Postischemic Stroke by Modulating Microglia Polarization. ACS nano. 2024 Jan 30;18(4):3260-3275.

[5]. Li F, et al. mRNA lipid nanoparticle-mediated pyroptosis sensitizes immunologically cold tumors to checkpoint immunotherapy. Nature communications. 2023 Jul 15;14(1):4223. [Content Brief]

[6]. Li Z, et al. Enzyme-Catalyzed One-Step Synthesis of Ionizable Cationic Lipids for Lipid Nanoparticle-Based mRNA COVID-19 Vaccines. ACS nano. 2022 Nov 22;16(11):18936-18950. [Content Brief]

[7]. Zhang C, et al. mRNA-Based Vaccination Drives in Vivo Dendritic Cell Reprogramming and Selective Cytotoxic T Lymphocyte Modulation for Enhanced Antitumor Immunity. ACS nano. 2025 Nov 11;19(44):38267-38283.

[8]. Xu Y. Rational design and combinatorial chemistry of ionizable lipids for RNA delivery. Journal of Materials Chemistry B. 2023 Jul 19;11(28):6527-39.

Calculators

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
Ethanol	1 mM	1.4304 mL	7.1521 mL	14.3041 mL	35.7603 mL
	5 mM	0.2861 mL	1.4304 mL	2.8608 mL	7.1521 mL
	10 mM	0.1430 mL	0.7152 mL	1.4304 mL	3.5760 mL
	15 mM	0.0954 mL	0.4768 mL	0.9536 mL	2.3840 mL
	20 mM	0.0715 mL	0.3576 mL	0.7152 mL	1.7880 mL
	25 mM	0.0572 mL	0.2861 mL	0.5722 mL	1.4304 mL
	30 mM	0.0477 mL	0.2384 mL	0.4768 mL	1.1920 mL
	40 mM	0.0358 mL	0.1788 mL	0.3576 mL	0.8940 mL
	50 mM	0.0286 mL	0.1430 mL	0.2861 mL	0.7152 mL
	60 mM	0.0238 mL	0.1192 mL	0.2384 mL	0.5960 mL

Bioz

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100 mg

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