ABD-3001
Based on 1 Customer Validation
ABD-3001 is an ALDH1 inhibitor. ABD-3001 induces costimulatory molecules, ER/protein homeostasis stress, and extracellular Calreticulin exposure compatible with immunogenic cell death. ABD-3001 can be used for research on acute undifferentiated leukemia, acute myeloid leukemia, and acute lymphoblastic leukemia.
For research use only. We do not sell to patients.
- Purity : 95.44%
- CAS No.: 350229-29-7
- Formula: C9H15NOS
- Molecular Weight:185.29
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
ALDH1 |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| DU-145 | IC50 |
6 μM
Compound: 2a, DIMATE
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Growth inhibition of human DU145 cells
Growth inhibition of human DU145 cells
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[PMID: 17692435] |
| DU-145 | IC50 |
7.2 μM
Compound: 2a, DIMATE
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Growth inhibition of human DU145 cells administered after 3 days
Growth inhibition of human DU145 cells administered after 3 days
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[PMID: 17692435] |
| MRC5 | IC50 |
8 μM
Compound: 2a, DIMATE
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Growth inhibition of human MRC5 cells
Growth inhibition of human MRC5 cells
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[PMID: 17692435] |
| RH7777 | IC50 |
10 μM
Compound: 2a, DIMATE
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Growth inhibition of RH7777 cells
Growth inhibition of RH7777 cells
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[PMID: 17692435] |
In Vitro
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:NSG (NOD.Cg-PrkdcSCID Il2rgtm1Wjl/SzJ, 6 weeks old)[3]
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Dosage:5 mg/kg
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Administration:intraperitoneal; three times per week; 24 days
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Result:Leukemia outgrowth was controlled over the course of dosing, except for one animal.
Leukemic burden expanded after dosing was stopped.
Animals showed an extension of survival.
Clinical Trial
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 350229-29-7
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Appearance Liquid (Density: 1.036±0.06 g/cm3)
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Molecular Weight 185.29
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Formula C9H15NOS
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Color Colorless to light yellow
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SMILES
O=C(SC)C#CC(C)(C)N(C)C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (539.69 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (13.49 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (13.49 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.3969 mL | 26.9847 mL | 53.9695 mL | 134.9236 mL |
| 5 mM | 1.0794 mL | 5.3969 mL | 10.7939 mL | 26.9847 mL | |
| 10 mM | 0.5397 mL | 2.6985 mL | 5.3969 mL | 13.4924 mL | |
| 15 mM | 0.3598 mL | 1.7990 mL | 3.5980 mL | 8.9949 mL | |
| 20 mM | 0.2698 mL | 1.3492 mL | 2.6985 mL | 6.7462 mL | |
| 25 mM | 0.2159 mL | 1.0794 mL | 2.1588 mL | 5.3969 mL | |
| 30 mM | 0.1799 mL | 0.8995 mL | 1.7990 mL | 4.4975 mL | |
| 40 mM | 0.1349 mL | 0.6746 mL | 1.3492 mL | 3.3731 mL | |
| 50 mM | 0.1079 mL | 0.5397 mL | 1.0794 mL | 2.6985 mL | |
| 60 mM | 0.0899 mL | 0.4497 mL | 0.8995 mL | 2.2487 mL | |
| 80 mM | 0.0675 mL | 0.3373 mL | 0.6746 mL | 1.6865 mL | |
| 100 mM | 0.0540 mL | 0.2698 mL | 0.5397 mL | 1.3492 mL |
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