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Cat. No.: HY-W747508

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Aculeacin A

Aculeacin A is a β-1,3-glucan synthase inhibitor. Aculeacin A is an antifungal antibiotic. Aculeacin A inhibits cell wall glucan synthesis in growing yeast cells, induces cell lysis, osmotic fragility, cell aggregation and abnormal cell morphology, without affecting the synthesis of proteins, nucleic acids or mannan. Aculeacin A acts only on growing yeast cells and has a narrow antifungal spectrum, mainly targeting Candida and Torulopsis species. Aculeacin A shows reduced activity in human serum and exhibits a paradoxical dose-response pattern. Aculeacin A has an inoculum effect against some Candida albicans strains, and its activity is pH-stable. Aculeacin A can be used in studies of fungal infections, such as those caused by Candida, Torulopsis and other species.

For research use only. We do not sell to patients.

CAS No.: 58814-86-1
Formula: C₅₀H₈₁N₇O₁₆
Molecular Weight:1036.22

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Aculeacin A Chemical Structure

CAS No.: 58814-86-1

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

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- In Vitro
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Description

IC₅₀ & Target

^[4]

Lipopeptide

All Antibiotic Isoforms :

In Vitro

Fungal InfectionViral Infection

Fungal Infection

Aculeacin A (0.05-0.5 μg/mL; up to 20 hours) completely inhibits the growth of Saccharomyces cerevisiae Hansen 0209 strain at a concentration of 0.5 μg/mL, and partially inhibits its growth at 0.2 μg/mL, when incubated in glucose-peptone medium for up to 20 hours^[2].

Fungal Infection

Aculeacin A (0.5-50 μg/mL; up to 4 h) exhibits fungicidal activity against actively growing cells of Saccharomyces cerevisiae Hansen 0209 strain, and reduces cell viability within 2 h of incubation, but has no effect on the viability of non-growing resting cells even at the highest concentration of 50 μg/mL^[2].

Fungal Infection

Aculeacin A (5 μg/mL; 1 hour) causes cell lysis at the budding tips of Saccharomyces cerevisiae Hansen 0209 strain within 1 hour of incubation in glucose-peptone medium^[2].

Fungal Infection

Aculeacin A (5 μg/mL; 10 minutes pre-incubation, followed by incubation for up to 90 minutes) inhibits the incorporation of [U-¹⁴C]glucose into acid-insoluble fractions of Saccharomyces cerevisiae Hansen 0209 cells, but exerts no effect on the incorporation of [1-¹⁴C]leucine or [2-¹⁴C]uridine after up to 90 minutes of incubation with the radioactive precursors^[2].

Fungal Infection

Aculeacin A (5 μg/mL; 10 minutes pre-incubation followed by 30 minutes treatment) selectively inhibits the incorporation of [U-¹⁴C] glucose into the glucan component of the cell wall of Saccharomyces cerevisiae Hansen 0209 strain, reducing the incorporation level to 45.7% of that in the control group, while exerting minimal effects on mannan synthesis^[2].

Fungal Infection

Aculeacin A (10-100 μg/mL) weakly induces protoplast lysis of Saccharomyces cerevisiae Hansen 0209 only at the relatively high concentration of 100 μg/mL, while no effect is observed at 10 μg/mL^[2].

Fungal Infection

Aculeacin A (5 μg/mL; 1 hour) induces membrane invagination and the formation of membranous spherical bodies in the gap between the cell wall and cytoplasm in intact Saccharomyces cerevisiae Hansen 0209 strain cells after 1 hour of incubation^[2].

Fungal Infection

The inhibitory activity of Aculeacin A against 6 strains of Candida albicans is almost unaffected by variations in medium pH within the range of 3.0 to 8.0^[3].

Fungal Infection

Aculeacin A (0.02-20 μg/mL; 6 hours) exhibits fungicidal activity against actively growing Candida albicans, Candida tropicalis, and Torulopsis glabrata, with its lethal activity peaking at concentrations ranging from 0.08 to 0.31 μg/mL, while higher concentrations reduce its fungicidal effect^[3].

Fungal Infection

Aculeacin A (0.02-20 μg/mL; up to 6 hours) exerts paradoxical fungicidal and growth-inhibitory effects on exponentially growing Candida albicans MTU 12077 cells, with activity peaking at 0.08~1.25 μg/mL and decreasing at higher concentrations^[4].

Fungal Infection

Studies show that Aculeacin A (0.31 μg/mL; 0-6 hours) exerts no effect on non-proliferating Candida albicans cells, but significantly reduces the viability of proliferating cells at the same concentration^[4].

Fungal Infection

Aculeacin A (0.08-1.25 μg/mL; 30 minutes) induces visible aggregates in exponentially growing susceptible yeast cells, including Candida albicans, but does not induce aggregation in non-susceptible yeast strains^[4].

Fungal Infection

Aculeacin A (0.08-20 μg/mL; 6 hours) induces concentration-dependent morphological changes in exponentially growing Candida albicans MTU 12077 cells. It causes cell lysis at concentrations ranging from 0.08 to 0.31 μg/mL, and induces abnormally large cell morphology at a concentration of 20 μg/mL^[4].

Fungal Infection

Aculeacin A (0.31-20 μg/mL; 3 hours) induces osmotically sensitive cells in Candida albicans MTU 12077, with lower concentrations resulting in greater sensitivity, while osmotic stabilization inhibits the drug-induced cell aggregation^[4].

Viral Infection

Aculeacin A (0.01-100 μg/mL; 20 minutes pre-incubation) preferentially inhibits the synthesis of alkali-insoluble glucan in osmotically stabilized exponential-phase Candida albicans MTU 12077 cells (with an inhibition rate of approximately 80% at 0.31 μg/mL), and exhibits an anomalous dose-response: the inhibitory effect reaches a peak at 0.31 μg/mL, while the inhibition weakens at higher concentrations^[4].

Fungal Infection

Aculeacin A (0.31-20 μg/mL; 3 hours) induces alkali-insoluble glucan-deficient Candida albicans MTU 12077 cells, with their glucan content reduced to ~20-23% of that in the control group, while other cellular components are minimally affected^[4].

Fungal Infection

Aculeacin A (0.08-80 μg/mL; 60 minutes) inhibits β-(1,3)-glucan synthase activity in cell-free extracts of Candida albicans MTU 12077 and Saccharomyces cerevisiae MTU 09018 (with maximum inhibition rates of up to 64% at 8 μg/mL and up to 75% at 0.8 μg/mL), and the inhibitory effect weakens at higher concentrations^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[2]

Cell Line:

Saccharomyces cerevisiae strain Hansen 0209 cells
Concentration:

0.05 μg/mL, 0.2 μg/mL, 0.5 μg/mL
Incubation Time:

up to 20 hours
Result:

Completely inhibited growth over the 20-hour incubation period at 0.5 μg/mL.
Partially reduced growth compared to untreated controls at 0.2 μg/mL.
Showed minimal growth inhibition relative to higher concentrations at 0.05 μg/mL.

Cell Viability Assay^[2]

Cell Line:

Saccharomyces cerevisiae strain Hansen 0209 cells
Concentration:

tested concentrations (growth-permissive medium); 5 μg/mL, 50 μg/mL (non-growth buffer)
Incubation Time:

up to 4 hours (growth-permissive medium); up to 4 hours (non-growth buffer)
Result:

Exerted a fungicidal effect at all tested concentrations in growth-permissive glucose-peptone medium, with a distinct decrease in viable cells occurring within the first 2 hours, followed by a gradual reduction in the rate of cell death.
Caused no decrease in viable cells at 5 μg/mL or 50 μg/mL over 4 hours in non-growth 0.1 M phosphate buffer.

Related Protocols :

Chemical Information

CAS No. 58814-86-1
Molecular Weight 1036.22
Formula C₅₀H₈₁N₇O₁₆
SMILES

CCCCCCCCCCCCCCCC(NC1C(NC(C(O)C)C(N2C(CC(O)C2)C(NC(C(O)C(O)C3=CC=C(O)C=C3)C(NC(C(O)C)C(N4C(C(O)C(C)C4)C(NC(O)C(O)C1)=O)=O)=O)=O)=O)=O)=O
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Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Zhang F, et al. Biochemical and genetic characterization of fungal proline hydroxylase in echinocandin biosynthesis. Applied microbiology and biotechnology. 2018 Sep;102(18):7877-7890. [Content Brief]

[2]. Mizoguchi J, et al. On the mode of action of a new antifungal antibiotic, aculeacin A: inhibition of cell wall synthesis in Saccharomyces cerevisiae. The Journal of antibiotics. 1977 Apr;30(4):308-13. [Content Brief]

[3]. Iwata K et al. In vitro studies of aculeacin A, a new antifungal antibiotic. J Antibiot (Tokyo). 1982 Feb;35(2):203-9. [Content Brief]

[4]. Yamaguchi H et al. Studies on the mechanism of antifungal action of aculeacin A. J Antibiot (Tokyo). 1982 Feb;35(2):210-9. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Biological Activity
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Chemical Information
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Aculeacin A

All Antibiotic Isoforms

Biological Activity

Description

IC50 & Target

In Vitro

Chemical Information

Purity & Documentation

References

Calculators

Help & FAQs

Aculeacin A Related Classifications

HY-W747508 Related Classifications

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