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Cat. No.: HY-189958

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AKT-IN-29

AKT-IN-29 is a potent Akt1 inhibitor with IC₅₀ = 0.209 μM. AKT-IN-29 inhibits this kinase by forming multiple stable interactions with the Akt1 active site, thereby disrupting the mitochondrial membrane potential and activating the caspase-3 cascade, ultimately inducing apoptosis via the intrinsic pathway. AKT-IN-29 can be used for research on breast cancer.

For research use only. We do not sell to patients.

Formula: C₂₂H₂₀ClN₅O₂S₃
Molecular Weight:518.07

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

AKT-IN-29 Chemical Structure

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

^[1]

Akt1

0.209 μM (IC₅₀)

Caspase-3

All Akt Isoforms :

All Caspase Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
MCF7	IC₅₀	10.186 μM	Cytotoxicity against human MCF-7 breast cancer cells measured by MTT assay after 24 hrs incubation. Cytotoxicity against human MCF-7 breast cancer cells measured by MTT assay after 24 hrs incubation.	42755039
MDA-MB-231	IC₅₀	24.411 μM	Cytotoxicity against human MDA-MB-231 breast cancer cells measured by MTT assay after 24 hrs incubation. Cytotoxicity against human MDA-MB-231 breast cancer cells measured by MTT assay after 24 hrs incubation.	42755039
NIH3T3	IC₅₀	60.974 μM	Cytotoxicity against mouse NIH/3T3 embryonic fibroblast cells measured by MTT assay after 24 hrs incubation. Cytotoxicity against mouse NIH/3T3 embryonic fibroblast cells measured by MTT assay after 24 hrs incubation.	42755039

In Vitro

Anti-cancerMitochondrial FunctionApoptosisOthers

Anti-cancer

AKT-IN-29 (compound 5h) (0.316-1000 μM; 24 h) exhibits potent antiproliferative effects in MCF-7 cells with an IC₅₀ of 10.186 μM and a selectivity index of 5.986 relative to NIH/3T3 cells, and shows moderate cytotoxic effects in MDA-MB-231 cells with an IC₅₀ value of 24.411 μM^[1].

Mitochondrial Function

Anti-cancer

Apoptosis

AKT-IN-29 (10.186 μM; 24 h) significantly induces apoptosis, causes depolarization of the mitochondrial membrane potential, and highly activates Caspase-3 in MCF-7 cells ^[1].

Others

AKT-IN-29 (1 nM-1 mM) selectively inhibits Akt1 in a cell-free Akt1 assay with an IC₅₀ of 0.209 μM, and shows no significant inhibitory effect on aromatase with an IC₅₀ > 10 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:

MCF-7 human breast cancer cells
Concentration:

10.186 μM
Incubation Time:

24 h
Result:

Significantly induced apoptosis in MCF-7 cells, with the total apoptotic cell population reaching 19.3% (predominantly late apoptosis at 18.3%), while the necrosis rate was extremely low, confirming that the anticancer effect is mediated by apoptosis.

Related Protocols :

Chemical Information

Molecular Weight 518.07
Formula C₂₂H₂₀ClN₅O₂S₃
SMILES

ClC1=CC=C(SC(SCC2=NN=C(SCC(N3CCOCC3)=O)N2C4=CC=CC=C4)=N5)C5=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Çiftçi B, et al. Synthesis, Structural Characterization, and Investigation of Biological Effects of Novel Benzothiazole-Triazole Derivatives Designed as AKT Kinase Inhibitors. Chemical biology & drug design. 2026 Sep;108(3):e70404.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

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MOQ

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100 mg

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References

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AKT-IN-29

All Akt Isoforms

All Caspase Isoforms