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Cat. No.: HY-D3423

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AM2-10

Name: AM2-10
Brand: MedChemExpress
SKU: HY-D3423
Availability: PreOrder

(Synonyms: FM 2-10FX)

AM2-10 (FM 2-10FX) is a Fluorescent dye used for liquid-phase endocytosis imaging. AM2-10 stains cell membranes to observe neurite length during NGF-induced cell differentiation.

For research use only. We do not sell to patients.

Formula: C₂₅H₄₁Cl₃N₄
Molecular Weight:503.98

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

AM2-10 Chemical Structure

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
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Biological Activity

Description

AM2-10 (FM 2-10FX) is a Fluorescent dye used for liquid-phase endocytosis imaging. AM2-10 stains cell membranes to observe neurite length during NGF-induced cell differentiation^[1].

In Vitro

Guide (The following is our recommended protocol. This protocol is for guidance only and should be modified according to your specific needs).

1. Stock Solution Preparation
1.1 Solvent: OptiMEM.
1.2 Concentration recommendation: 20 μM.

3. Staining Procedure
3.1 Sample type: Adherent cells (PC12 cells grown on poly-L-lysine coverslips).[1]
3.2 Incubation conditions: Incubate cells with 20 μM AM2-10 for 5 min at 37°C; or image cells in solution containing AM2-10 for NGF-induced differentiation assessment.
3.3 Washing steps: Wash cells twice with ice-cold PBS.

5. Detection & Analysis
5.1 Instrument: Fluorescence microscope.
5.2 Result analysis:
5.2.1 Fluorescence localization: Stains all cellular membranes, including endocytic vesicles and neurite membranes.
5.2.2 Result application: Used to assess fluid-phase endocytosis efficiency and NGF-induced neurite differentiation in PC12 cells.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Related Protocols :

Chemical Information

Molecular Weight 503.98
Formula C₂₅H₄₁Cl₃N₄
SMILES

CCN(CC)C1=CC=C(/C=C/C2=CC=[N+](CCC[N+](C)(C)CCC[NH3+])C=C2)C=C1.[Cl-].[Cl-].[Cl-]
Synonyms

FM 2-10FX
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Bethani I, et al. Endosomal fusion upon SNARE knockdown is maintained by residual SNARE activity and enhanced docking. Traffic (Copenhagen, Denmark). 2009 Oct;10(10):1543-59.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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