Cat. No.: HY-N8912

Ambrosin

Ambrosin is an orally active NF-κβ and BACE1 inhibitor. Ambrosin reduces pro-inflammatory and nitrosative mediators, increases PPARγ levels, inhibits apoptosis (Apoptosis), enhances autophagy (Autophagy), and ameliorates oxidative stress. Ambrosin reduces Amyloid plaque deposition. Ambrosin improves lipopolysaccharide-induced memory impairment and colonic histopathological changes. Ambrosin can be used in research related to Alzheimer's disease and colitis.

For research use only. We do not sell to patients.

CAS No.: 509-93-3
Formula: C₁₅H₁₈O₃
Molecular Weight:246.31

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Ambrosin Chemical Structure

CAS No.: 509-93-3

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

^[2]^[1]

PPAR-γ

BACE1

All NF-κB Isoforms :

All Beta-secretase Isoforms :

BACE1
BACE2

All PPAR Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	GI₅₀	1.8 μM Compound: 1	The compound was evaluated for cytotoxicity against colon cancer cell line HCT116 The compound was evaluated for cytotoxicity against colon cancer cell line HCT116	[PMID: 7650694]
KB	ED₅₀	0.45 μg/mL Compound: 12. Ambrosin	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 17336532]
KB	ED₅₀	1.83 μM Compound: ambrosin	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 18329753]
LOX IMVI	GI₅₀	2.2 μM Compound: 1	The compound was evaluated for cytotoxicity against melanoma cell line LOX-IMVI The compound was evaluated for cytotoxicity against melanoma cell line LOX-IMVI	[PMID: 7650694]
LOX IMVI	GI₅₀	2.2 μM Compound: 6	Antiproliferative activity against human LOXIMVI cells Antiproliferative activity against human LOXIMVI cells	10.1039/C2MD20172K
MCF7	GI₅₀	3.9 μM Compound: 1	The compound was evaluated for cytotoxicity against breast cancer cell line MCF-7 The compound was evaluated for cytotoxicity against breast cancer cell line MCF-7	[PMID: 7650694]
MCF7	GI₅₀	3.9 μM Compound: 6	Antiproliferative activity against human MCF7 cells Antiproliferative activity against human MCF7 cells	10.1039/C2MD20172K
NCI-H522	GI₅₀	1 μM Compound: 1	The compound was evaluated for cytotoxicity against lung cancer cell line NCI-H522 The compound was evaluated for cytotoxicity against lung cancer cell line NCI-H522	[PMID: 7650694]
NCI-H522	GI₅₀	1 μM Compound: 6	Antiproliferative activity against human NCI-H522 cells Antiproliferative activity against human NCI-H522 cells	10.1039/C2MD20172K
OVCAR-5	GI₅₀	4.9 μM Compound: 1	The compound was evaluated for cytotoxicity against ovarian cancer cell line OVCAR-5 The compound was evaluated for cytotoxicity against ovarian cancer cell line OVCAR-5	[PMID: 7650694]
PC-3	GI₅₀	4.5 μM Compound: 1	The compound was evaluated for cytotoxicity against prostate cancer cell line PC-3 The compound was evaluated for cytotoxicity against prostate cancer cell line PC-3	[PMID: 7650694]
SF-539	GI₅₀	2.9 μM Compound: 1	The compound was evaluated for cytotoxicity against CNS cancer cell line CNS SF-539 The compound was evaluated for cytotoxicity against CNS cancer cell line CNS SF-539	[PMID: 7650694]
SN12C	GI₅₀	3.4 μM Compound: 1	The compound was evaluated for cytotoxicity against renal cancer cell line SN12C The compound was evaluated for cytotoxicity against renal cancer cell line SN12C	[PMID: 7650694]

In Vitro

OthersInnate Immunity

Others

Ambrosin exhibits predicted high gastrointestinal absorption, favorable lipid solubility, and robust BBB permeability, indicating superior pharmacokinetic properties for central nervous system targeting compared to curcumin^[1].

Innate Immunity

Ambrosin binds to the NF-κβp65-DNA complex via hydrogen bonding and hydrophobic interactions, indicating reversible inhibition of NF-κβp65 activation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Innate ImmunityCytokines & ChemokinesAβ/Tau/α-Synuclein AggregationInflammasomeOxidative StressAutophagyApoptosis

Innate Immunity

Cytokines & Chemokines

Aβ/Tau/α-Synuclein Aggregation

Ambrosin (5-10 mg/kg; i.p.; daily; 7 days) ameliorates LPS-induced Alzheimer's disease-like pathology in mice by inhibiting NF-κβp65 and downstream mediators, improving memory function, reducing neuroinflammation, decreasing amyloidogenesis, and protecting neurons, with the 10 mg/kg dose showing greater efficacy^[1].

Innate Immunity

Cytokines & Chemokines

Inflammasome

Oxidative Stress

Autophagy

Apoptosis

Ambrosin (10 mg/kg; p.o.) significantly mitigates DSS-induced colitis in male BALB/c mice by restoring antioxidant balance, suppressing pro-inflammatory signaling pathways, enhancing autophagy, and inhibiting apoptosis^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Albino mice (mature male, 120-130 g, LPS-induced Alzheimer's disease model)^[1]
Dosage:

5 mg/kg; 10 mg/kg
Administration:

i.p.; daily; 7 days
Result:

Reduced mean escape latency time on days 3 and 4 to levels similar to the normal group (significantly lower than LPS control).
Increased mean time spent in the target quadrant to 25.59 sec (5 mg/kg) and 25.56 sec (10 mg/kg; LPS control: 12.20 sec; normal group: 23.38 sec).
Increased time spent exploring the novel object vs. the familiar object, and yielded significantly positive discrimination indices (significantly different from LPS control with negative index).
Reduced LPS-induced elevated NF-κβp65 transcript and protein levels in a dose-dependent manner, with 10 mg/kg reducing protein levels to values not significantly different from the normal group.
Significantly reduced LPS-induced elevated TNF-α, IL-1β, COX-2, and iNOS protein levels at 5 mg/kg; reduced these markers to levels similar to the normal group at 10 mg/kg.
Reduced LPS-induced elevated BACE1 protein levels in a dose-dependent manner.
Increased surviving hippocampal CA1 neurons to 35.66 per high power field (5 mg/kg; LPS control: 23.33) and 44.33 per high power field (10 mg/kg).
Reduced amyloid plaques to 2.50 per high power field (5 mg/kg; LPS control: 6.20) and 0.90 per high power field (10 mg/kg).

Animal Model:

BALB/c (male, 16-26 g, DSS-induced colitis)^[2]
Dosage:

10 mg/kg
Administration:

p.o.
Result:

Significantly reduced disease activity index (DAI).
Restored colon length compared to DSS-only treated mice.
Increased colonic tissue levels of SIRT1, PPAR-γ, glutathione reductase, glutathione peroxidase, Nrf2, beclin-1, LC3-II, and Bcl-2.
Significantly decreased colonic tissue levels of ROS, iNOS, TLR-4, IL-1β, IL-6, TGF-β1, phospho-p38 MAPK, c-Fos, c-Jun, NLRP3 inflammasome, and caspase-8.
Attenuated DSS-induced histopathological damage, including reduced ulceration, crypt loss, and inflammatory cell infiltration.
Lowered histological disease score.
Reduced NF-κB (p65) nuclear immunostaining.
Improved electron microscopic features of colonic cells (nearly normal apical microvilli, mild cytoplasmic vacuolation, and mild mitochondrial swelling with partially destructed cristae).

Related Protocols :

Chemical Information

CAS No. 509-93-3
Molecular Weight 246.31
Formula C₁₅H₁₈O₃
SMILES

C[C@]12[C@]3([C@](C(=C)C(=O)O3)(CC[C@H](C)[C@@]1(C=CC2=O)[H])[H])[H]
Structure Classification
- Terpenoids
- Diterpenoids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Khalil MNA, et al. Ambrosin, a potent NF-κβ inhibitor, ameliorates lipopolysaccharide induced memory impairment, comparison to curcumin. PloS one. 2019;14(7):e0219378. [Content Brief]

[2]. Kabel AM, et al. Perindopril/Ambrosin Combination Mitigates Dextran Sulfate Sodium-Induced Colitis in Mice: Crosstalk between Toll-Like Receptor 4, the Pro-Inflammatory Pathways, and SIRT1/PPAR-γ Signaling. Pharmaceuticals (Basel, Switzerland). 2022 May 13;15(5):600. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Ambrosin

All NF-κB Isoforms

All Beta-secretase Isoforms

All PPAR Isoforms