Cat. No.: HY-190499

Anti-inflammatory agent 128

Anti-inflammatory agent 128 is a 2-azido-3-O-benzyl-2-deoxy sp²-iminosugar glycolipid with anti-inflammatory, antitumor, and antiparasitic activities. Anti-inflammatory agent 128 exhibits broad-spectrum antiproliferative activity against multiple human tumor cell lines. Anti-inflammatory agent 128 elevates mitochondrial ROS levels in Leishmania donovani promastigotes. Anti-inflammatory agent 128 inhibits the inflammatory response of Bv.2 microglia under LPS stimulation, suppresses NLRP3 inflammasome activation, downregulates the pro-inflammatory mediators Tnfa, Il1β, and Il6, and upregulates Hmox1. Anti-inflammatory agent 128 attenuates reactive gliosis in isolated retinal explants from diabetic rats and ameliorates inflammation-related phenotypes associated with diabetic retinopathy. Anti-inflammatory agent 128 can be used in research on tumors, leishmaniasis, and diabetic retinopathy.

For research use only. We do not sell to patients.

Formula: C₂₆H₄₀N₄O₆S
Molecular Weight:536.68

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Anti-inflammatory agent 128 Chemical Structure

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

^[1]

NLRP3

IL-6

IL-8

Caspase-1

TNF-α

All Parasite Isoforms :

All NOD-like Receptor (NLR) Isoforms :

All Interleukin Related Isoforms :

All Caspase Isoforms :

All TNF Receptor Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	GI50	8.5 μM	Antiproliferative activity against human A549 lung cancer cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay. Antiproliferative activity against human A549 lung cancer cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay.	42828963
HeLa	GI50	2.8 μM	Antiproliferative activity against human HeLa cervix cancer cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay. Antiproliferative activity against human HeLa cervix cancer cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay.	42828963
MIA PaCa-2	GI50	9.2 μM	Antiproliferative activity against human MIA PaCa-2 pancreatic cancer cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay. Antiproliferative activity against human MIA PaCa-2 pancreatic cancer cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay.	42828963
SW1573	GI50	8.9 μM	Antiproliferative activity against human SW1573 lung cancer cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay. Antiproliferative activity against human SW1573 lung cancer cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay.	42828963
T47D	GI50	5.4 μM	Antiproliferative activity against human T-47D breast cancer cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay. Antiproliferative activity against human T-47D breast cancer cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay.	42828963
WiDr	GI50	3.6 μM	Antiproliferative activity against human WiDr colon cancer cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay. Antiproliferative activity against human WiDr colon cancer cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay.	42828963
THP-1	IC50	11.66 μM	Inhibition of intracellular Leishmania major amastigote growth in THP-1 macrophage-differentiated cells after 72 hrs by luciferase assay. Inhibition of intracellular Leishmania major amastigote growth in THP-1 macrophage-differentiated cells after 72 hrs by luciferase assay.	42828963
MRC5	CC₅₀	35.11 μM	Cytotoxicity against human MRC-5 lung fibroblasts assessed as viability reduction after 72 hrs by resazurin assay. Cytotoxicity against human MRC-5 lung fibroblasts assessed as viability reduction after 72 hrs by resazurin assay.	42828963
THP-1	CC₅₀	37.98 μM	Cytotoxicity against human THP-1 macrophages assessed as viability reduction after 72 hrs by resazurin assay. Cytotoxicity against human THP-1 macrophages assessed as viability reduction after 72 hrs by resazurin assay.	42828963

In Vitro

Anti-cancerCytokines & ChemokinesInflammasomeInnate ImmunityOxidative StressParasitic InfectionOthers

Anti-cancer

Anti-inflammatory agent 128 (8-NJ) (1-100 µM; 48 h) inhibits the proliferation of A549, HeLa, MIA PaCa-2, SW1573, T-47D and WiDr cells, with GI₅₀ values of 8.5, 2.8, 9.2, 8.9, 5.4 and 3.6 µM, respectively^[1].

Cytokines & Chemokines

Inflammasome

Anti-inflammatory agent 128 (20 µM; 24 h) decreases Tnfa, Il6, Il1b, and Nlrp3 mRNA and increases Hmox1 mRNA, and reduces GFAP immunoreactivity in BB rat retinal explants^[1].

Innate Immunity

Anti-inflammatory agent 128 (1-10 µM; preincubation for 3 h) reduces nitrite release from LPS-stimulated Bv.2 microglia^[1].
Anti-inflammatory agent 128 (10 µM) reduces the maturation of caspase-1 and IL-1β in LPS-stimulated Bv.2 microglia^[1].

Innate Immunity

Cytokines & Chemokines

Anti-inflammatory agent 128 (10 µM; 24 h) attenuates LPS-induced Tnfa, Il6, and Il1b mRNA expression in Bv.2 microglia and reduces NLRP3 mRNA expression and protein levels^[1].

Oxidative Stress

Parasitic Infection

Anti-inflammatory agent 128 (50 µM; 30 min) increases mitochondrial ROS in L. donovani promastigotes^[1].

Parasitic Infection

Anti-inflammatory agent 128 inhibits intracellular L. donovani and L. major amastigotes with IC₅₀ values of 8.79 and 11.66 µM, respectively, and exhibits CC₅₀ values of 35.11 µM in MRC-5 cells and 37.98 µM in THP-1 cells^[1].

Others

Anti-inflammatory agent 128 (0.1-10 µM; 24 h) does not reduce Bv.2 microglial cell viability^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

Bv.2 microglial cells
Concentration:

0.1, 0.5, 1.0, 5.0, and 10 µM
Incubation Time:

24 h
Result:

Cell survival was not compromised at any tested concentration after 24 h.

Real Time qPCR^[1]

Cell Line:

Bv.2 microglial cells
Concentration:

10 µM
Incubation Time:

24 h
Result:

Attenuated LPS-induced Tnfa, Il6, and Il1b mRNA expression in Bv.2 microglia.

Western Blot Analysis^[1]

Cell Line:

Bv.2 microglial cells
Concentration:

10 µM
Incubation Time:

24 h
Result:

Reduced LPS-induced NLRP3 protein levels.

Related Protocols :

Chemical Information

Molecular Weight 536.68
Formula C₂₆H₄₀N₄O₆S
SMILES

O=C1N2[C@H](S(=O)(CCCCCCCCCCCC)=O)[C@H](N=[N+]=[N-])C(OCC3=CC=CC=C3)[C@H](O)[C@H]2CO1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Rodríguez-Marín R, et al. Nitrogen-functionalized 2-deoxy sp2-iminoglycolipids: Stereoselective synthesis and biological evaluation. Eur J Med Chem. 2026 Oct 1;321(Pt 1):119392.

Calculators

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Anti-inflammatory agent 128

All Parasite Isoforms

All NOD-like Receptor (NLR) Isoforms

All Interleukin Related Isoforms

All Caspase Isoforms

All TNF Receptor Isoforms