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Cat. No.: HY-189411

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Apoptosis inducer 65

Apoptosis inducer 65 is an apoptosis inducer with antiproliferative activity against cancer cells. Apoptosis inducer 65 induces G0/G1 cell cycle arrest and intrinsic apoptosis by upregulating p53, BAX, and caspase-7, while downregulating Bcl-2. Apoptosis inducer 65 inhibits ROS, NO, TNF-α, IL-1β, and IL-6 in macrophages. Apoptosis inducer 65 can be used for research on hepatocellular carcinoma and inflammation.

For research use only. We do not sell to patients.

Formula: C₂₆H₁₈Br₃N₃O₄
Molecular Weight:676.15

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Apoptosis inducer 65 Chemical Structure

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

^[1]

IL-6

IL-1β

Bax

Caspase-7

Bcl-2

All Caspase Isoforms :

All Bcl-2 Family Isoforms :

All TNF Receptor Isoforms :

All Interleukin Related Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HepG2	IC50	7.81 μM	Cytotoxicity against human HepG-2 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay. Cytotoxicity against human HepG-2 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay.	42630597
HCT-116	IC50	15.34 μM	Cytotoxicity against human HCT-116 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay. Cytotoxicity against human HCT-116 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay.	42630597
MCF7	IC50	44.66 μM	Cytotoxicity against human MCF-7 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay. Cytotoxicity against human MCF-7 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay.	42630597
PANC-1	IC50	63.24 μM	Cytotoxicity against human Panc-1 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay. Cytotoxicity against human Panc-1 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay.	42630597
WI-38	IC50	67.2 μM	Cytotoxicity against normal human WI-38 fibroblasts assessed as reduction in cell viability incubated for 24 hrs by MTT assay. Cytotoxicity against normal human WI-38 fibroblasts assessed as reduction in cell viability incubated for 24 hrs by MTT assay.	42630597

In Vitro

Anti-cancerCell Cycle RegulationApoptosisInnate ImmunityCytokines & Chemokines

Anti-cancer

Apoptosis inducer 65 (Compound 9) (24 h) exhibits the highest antiproliferative activity against HepG-2 cells with an IC₅₀ of 7.81 μM, and shows the most favorable selectivity toward HepG2 cells with an SI of 7.81^[1].

Cell Cycle Regulation

Apoptosis inducer 65 (7.81 μM; 24 h) induces cell cycle arrest at the G0/G1 phase in HepG2 cells, increasing the cell population from 54.39% to 86.21%^[1].

Apoptosis

Apoptosis inducer 65 (7.81 μM; 24 h) induces apoptosis in HepG2 cells, increasing the total apoptotic cell population from 3.12% to 35.14%^[1].
Apoptosis inducer 65 (7.81 μM; 24 h) triggers apoptosis in HepG2 cells through activation of a p53-dependent pathway, upregulating p53 (5.89-fold) and BAX (3.71-fold) while downregulating Bcl-2 (0.64-fold)^[1].

Innate Immunity

Apoptosis inducer 65 (12.5-200 μM; 2 h pretreatment; 24 h incubation) exhibits low to moderate cytotoxicity in RAW 264.7 cells across the tested concentration range under basal and inflammatory conditions^[1].

Innate Immunity

Cytokines & Chemokines

Apoptosis inducer 65 (2.5-80 μM; 2 h pretreatment; 24 h incubation) effectively attenuates LPS (HY-D1056)-induced oxidative stress in RAW 264.7 macrophages and reduces ROS levels^[1].
Apoptosis inducer 65 (10 μM; 24 h) inhibits the expression of pro-inflammatory cytokines in LPS-stimulated RAW 264.7 macrophages, reducing TNF-α to 0.36-fold, IL-1β to 0.47-fold, and IL-6 to 0.51-fold^[1].

Cytokines & Chemokines

Innate Immunity

Apoptosis inducer 65 (10 μM; 2 h pretreatment; 24 h incubation) strongly inhibits nitric oxide production in LPS-stimulated RAW 264.7 macrophages, reducing NO levels to 0.409-fold^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:

HepG2
Concentration:

7.81 μM
Incubation Time:

24 h
Result:

Increased the total apoptotic population (early + late) from 3.12% to 35.14%.
Increased late apoptosis from 0.18% to 23.27%.
Increased early apoptosis from 0.65% to 3.69%.
Increased necrotic cell death from 2.29% to 8.18%.

Real Time qPCR^[1]

Cell Line:

HepG2
Concentration:

7.81 μM
Incubation Time:

24 h
Result:

Upregulated p53 expression to 5.89-fold relative to control.
Upregulated BAX expression to 3.71-fold.
Downregulated Bcl-2 expression to 0.64-fold.
Increased the BAX/Bcl-2 ratio by 5.8-fold.
Elevated cytochrome c (CYC) expression to 3.94-fold.
Upregulated caspase-7 expression to 2.65-fold.

Cell Viability Assay^[1]

Cell Line:

RAW 264.7
Concentration:

12.5, 25, 50, 100, 150, 200 μM
Incubation Time:

2 h pretreatment; 24 h incubation
Result:

In the absence of LPS, maintained viability of 98.3%, 96.7%, and 98.6% at 12.5, 25, and 50 μM, respectively, and 83.6%, 75.4%, and 48.1% at 100, 150, and 200 μM, respectively.
Under LPS-stimulated conditions, maintained viability of 97.1%, 93.8%, and 96.4% at 12.5, 25, and 50 μM, respectively, and 76.3%, 60.7%, and 37.2% at 100, 150, and 200 μM, respectively.

Real Time qPCR^[1]

Cell Line:

RAW 264.7
Concentration:

10 μM
Incubation Time:

24 h
Result:

Reduced TNF-α expression to 0.36-fold relative to LPS-stimulated control cells.
Decreased IL-1β expression to 0.47-fold.
Reduced IL-6 levels to 0.51-fold.

Related Protocols :

Chemical Information

Molecular Weight 676.15
Formula C₂₆H₁₈Br₃N₃O₄
SMILES

BrC1=C(O)C(Br)=CC(/C(N2C3=CC=CC=C3NC(C4=CC(Br)=CC=C4O)=C2)=C(C(OCC)=O)\C#N)=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Alshawwa SZ, et al. Quinoxaline-based cyanoacrylate hybrids as multi-target anti-proliferative and anti-inflammatory agents with potent activity against hepatocellular carcinoma: induction of apoptosis and modulation of oxidative stress signaling. RSC advances. 2026 Sep 16;16(41):49178-49197.

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

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100 mg

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References

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Apoptosis inducer 65

All Caspase Isoforms

All Bcl-2 Family Isoforms

All TNF Receptor Isoforms

All Interleukin Related Isoforms