ATTO 488

Guide (The following is the experimental plan we recommend. This plan serves only as a reference guide. The specific operations should be adjusted according to your actual needs.)
1. Preparation of ATTO 488 Staining Solution : Prepare a 10 mM stock solution using DMSO.
2. Marking Process :
Add 2 times the molar amount of excess ATTO 488 to the purified protein.
Note: Conduct marking and spectral measurements in a green-safe light or dark environment, and perform the operation as quickly as possible.
3. Incubation Method: Incubate at room temperature (RT) for 2 hours or at 4°C in the dark with shaking for overnight.
4. Purification : 1) Use the NAP-5 size exclusion column to remove the free ATTO 488.
2) Remove trace free ATTO 488 through ultrafiltration.
5. Characterize the labeled protein using ultraviolet/visible spectroscopy.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.