Bacterial Sortase Substrate III, Abz/DNP
Based on 1 publication(s) in Google Scholar
Bacterial Sortase Substrate III, Abz/DNP is a fluorescent peptide substrate. Bacterial Sortase Substrate III, Abz/DNP undergoes cleavage catalyzed by Staphylococcus aureus sortase A (SrtAΔN24) and Streptococcus pyogenes sortase A (SrtAΔN81), and catalyzes the formation of an amide bond between the carboxyl group of threonine and the amino group of the cell wall cross-bridge. Cleavage of this substrate can be detected at Ex/Em=320 nm/420 nm.
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- CAS No.: 2857862-12-3
- Formula: C41H57N11O14
- Molecular Weight:927.96
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Bacterial Sortase Substrate III, Abz/DNP
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Biological Activity
Description
In Vitro
Bacterial Sortase Substrate III, Abz/DNP is catalyzed for hydrolysis by purified recombinant Staphylococcus aureus SrtAΔN24 with a Km of 17.5 μM[1].
Bacterial Sortase Substrate III, Abz/DNP serves as a substrate for purified recombinant Streptococcus pyogenes SrtAΔN81-catalyzed transpeptidation with Gly3, generating detectable products via HPLC[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2857862-12-3
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Appearance Solid
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Molecular Weight 927.96
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Formula C41H57N11O14
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Sequence
{Abz}-Leu-Pro-Glu-Thr-Gly-{Lys(Dnp)}-NH2
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Sequence Shortening
{Abz}-LPETG-{Lys(Dnp)}-NH2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (1)
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Journal Impact Factor
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Most Recent
Solvent & Solubility
In Vitro:
DMSO : 25 mg/mL (26.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.0776 mL | 5.3882 mL | 10.7763 mL | 26.9408 mL |
| 5 mM | 0.2155 mL | 1.0776 mL | 2.1553 mL | 5.3882 mL | |
| 10 mM | 0.1078 mL | 0.5388 mL | 1.0776 mL | 2.6941 mL | |
| 15 mM | 0.0718 mL | 0.3592 mL | 0.7184 mL | 1.7961 mL | |
| 20 mM | 0.0539 mL | 0.2694 mL | 0.5388 mL | 1.3470 mL | |
| 25 mM | 0.0431 mL | 0.2155 mL | 0.4311 mL | 1.0776 mL |
Bacterial Sortase Substrate III, Abz/DNP Related Classifications
HY-P1883 Related Classifications
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Keywords
- Bacterial Sortase Substrate III, Abz/DNP
- 2857862-12-3
- Fluorescent Dye
- purified recombinant Staphylococcus aureus SrtAΔN24
- Staphylococcus aureus sortase A (SrtAΔN24)
- fluorogenic peptide substrate
- staphylococcus aureus infection
- sortase-catalyzed hydrolysis
- purified recombinant Streptococcus pyogenes SrtAΔN81
- Streptococcus pyogenes sortase A (SrtAΔN81)
- transpeptidation reactions
- Inhibitor
- inhibitor
- inhibit