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Cat. No.: HY-P1889A

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Bim BH3, Peptide IV TFA

Bim BH3, Peptide IV TFA is an Aβ₄₂-binding peptide and the pro-apoptotic BH3 domain of the BIM protein. Bim BH3, Peptide IV TFA binds Aβ₄₂ with a K_d of 7.1 μM. Bim BH3, Peptide IV TFA modulates Aβ₄₂ structure, fibrillization pathways, aggregate morphology, and membrane interactions, inhibiting fibril formation while enhancing protofibril assembly. Bim BH3, Peptide IV TFA promotes Aβ₄₂ β-sheet formation and accelerates aggregation. Bim BH3, Peptide IV TFA enhances Aβ₄₂ membrane internalization and bilayer stiffening. Bim BH3, Peptide IV TFA induces neuronal cell death by enhancing BH3-induced membrane interactions of Aβ₄₂ prefibrillar species. Bim BH3, Peptide IV TFA can be used for research on Alzheimer's disease.

For research use only. We do not sell to patients.

Formula: C₁₄₅H₂₂₂N₄₄O₄₁S.xC₂HF₃O₂
Molecular Weight:3269.65 (free acid)

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Bim BH3, Peptide IV TFA Chemical Structure

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
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Biological Activity

Description

IC₅₀ & Target

^[1]

Aβ42

7.1 μM (Kd)

Bim

All Bcl-2 Family Isoforms :

In Vitro

Aβ/Tau/α-Synuclein AggregationApoptosis

Aβ/Tau/α-Synuclein Aggregation

Bim BH3, Peptide IV (5-20 μM) TFA binds to Aβ42 monomers with a K_d of 7.1 x 10^-6 M^[1].
Bim BH3, Peptide IV (14 hrs) TFA enhances Aβ₄₂ aggregation and fibrillation in a concentration-dependent manner^[1].
Bim BH3, Peptide IV (14 h) TFA alters the morphology and increases the abundance of Aβ₄₂ aggregates, forming smaller and thicker fibrillar species^[1].
Bim BH3, Peptide IV (1:0.5-1:1 mole ratio, 12.5-25 μM; 0-12 h) TFA accelerates the random coil-to-β sheet structural transformation of Aβ42 in a concentration-dependent manner^[1].
Bim BH3, Peptide IV (12.5-25 μM; 10 min-48 h) TFA significantly modulates Aβ₄₂ fibril assembly, promoting the formation of abundant pre-fibrillar and protofibril structures while inhibiting the formation of mature fibrils^[1].

Apoptosis

Bim BH3, Peptide IV (15-45 μM; 48 h) TFA enhances Aβ₄₂-induced toxicity in SH-SY5Y cells in a concentration-dependent manner, reducing cell viability to around 40%^[1].

Aβ/Tau/α-Synuclein Aggregation

Apoptosis

Bim BH3, Peptide IV (15 μM) TFA enhances Aβ42-induced morphological changes associated with cell death in SH-SY5Y cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:

SH-SY5Y neuroblastoma cell line
Concentration:

15 μM; 30 μM; 45 μM
Incubation Time:

48 hours
Result:

Decreased cell viability to around 40% at the highest BIM-BH3 concentration.
Significantly increased the fractions of apoptotic and necrotic cells.
Did not produce toxic effects when the BIM-BH3 peptide was alone.

Related Protocols :

Chemical Information

Molecular Weight 3269.65 (free acid)
Formula C₁₄₅H₂₂₂N₄₄O₄₁S.xC₂HF₃O₂
Sequence

Asp-Met-Arg-Pro-Glu-Ile-Trp-Ile-Ala-Gln-Glu-Leu-Arg-Arg-Ile-Gly-Asp-Glu-Phe-Asn-Ala-Tyr-Tyr-Ala-Arg-Arg
Sequence Shortening

DMRPEIWIAQELRRIGDEFNAYYARR
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (269 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Malishev R, et al. Interactions between BIM Protein and Beta-Amyloid May Reveal a Crucial Missing Link between Alzheimer's Disease and Neuronal Cell Death. ACS chemical neuroscience. 2019 Aug 21;10(8):3555-3564.

Calculators

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

Minimum order quantity

100 mg

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