Alpha-agarase
Definition:
EC Tree > 3 Hydrolases > 3.2 Glycosylases > 3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds > 3.2.1.158 alpha-agarase
Alpha-agarase is a Ca2+-requiring marine endo-glycosidase from Thalassomonas sp. that endohydrolyses (1->3)-alpha-L-galactosidic linkages in agarose, yielding agarotetraose as the major product; it can use agarose, agarohexaose and neoagarohexaose as substrates, agarohexaose hydrolysis gives dimers and tetramers with agarotetraose predominant, neoagarohexaose gives two types of trimer, and it also hydrolyses the highly sulfated agarose porphyran very efficiently but cannot hydrolyse kappa-carrageenan (EC 3.2.1.83) or iota-carrageenan (EC 3.2.1.157) (cf. beta-agarase EC 3.2.1.81). By cleaving the alpha-1,3 linkages it produces agaro-oligosaccharides used in marine biotechnology. It appears in viruses and cellular organisms.
Alpha-agarase is a Ca2+-requiring marine endo-glycosidase from Thalassomonas sp. that endohydrolyses (1->3)-alpha-L-galactosidic linkages in agarose, yielding agarotetraose as the major product; it can use agarose, agarohexaose and neoagarohexaose as substrates, agarohexaose hydrolysis gives dimers and tetramers with agarotetraose predominant, neoagarohexaose gives two types of trimer, and it also hydrolyses the highly sulfated agarose porphyran very efficiently but cannot hydrolyse kappa-carrageenan (EC 3.2.1.83) or iota-carrageenan (EC 3.2.1.157) (cf. beta-agarase EC 3.2.1.81). By cleaving the alpha-1,3 linkages it produces agaro-oligosaccharides used in marine biotechnology. It appears in viruses and cellular organisms.
References:
Biomedical Dictionary
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