E1 NEDD8-activating enzyme
Definition:
EC Tree > 6 Ligases > 6.2 Forming carbon-sulfur bonds > 6.2.1 Acid-thiol ligases > 6.2.1.64 E1 NEDD8-activating enzyme
E1 NEDD8-activating enzyme activates the ubiquitin-like protein NEDD8 in an ATP-dependent manner: ATP plus NEDD8 plus the E1 L-cysteine yields AMP plus diphosphate plus E1-S-(NEDD8)-yl-L-cysteine. NEDD8 conjugation (neddylation) activates cullin-RING ligase (CRL) E3 complexes, which cannot bind substrate directly and instead assemble on cullin scaffolds with substrate-recognition modules. Like ubiquitin, NEDD8 is first adenylated and then transferred to the catalytic cysteine of E1. Structurally it is a heterodimeric NEDD8 E1 (APP-BP1/UBA3).
E1 NEDD8-activating enzyme activates the ubiquitin-like protein NEDD8 in an ATP-dependent manner: ATP plus NEDD8 plus the E1 L-cysteine yields AMP plus diphosphate plus E1-S-(NEDD8)-yl-L-cysteine. NEDD8 conjugation (neddylation) activates cullin-RING ligase (CRL) E3 complexes, which cannot bind substrate directly and instead assemble on cullin scaffolds with substrate-recognition modules. Like ubiquitin, NEDD8 is first adenylated and then transferred to the catalytic cysteine of E1. Structurally it is a heterodimeric NEDD8 E1 (APP-BP1/UBA3).
Biomedical Dictionary
The Biomedical Dictionary is a comprehensive and professional collection of biological academic terms and subject datas. All explanations are supported by authoritative books or high impact factor literatures, and you can acquire accurate explanations of the biomedical terms you want to know.
Molarity Calculator
Dilution Calculator
