Fruit bromelain
Definition:
EC Tree > 3 Hydrolases > 3.4 Acting on peptide bonds (peptidases) > 3.4.22 Cysteine endopeptidases > 3.4.22.33 Fruit bromelain
Fruit bromelain is a cysteine endopeptidase from the pineapple plant Ananas comosus, scarcely inhibited by chicken cystatin. It hydrolyzes proteins with broad specificity for peptide bonds; Bz-Phe-Val-Arg-NHMec is a good synthetic substrate, but it shows no action on Z-Arg-Arg-NHMec, unlike stem bromelain. A related enzyme, pinguinain, is obtained from Bromelia pinguin but is inhibited by chicken cystatin. In the fruit it contributes to ripening and protein mobilization. It features a papain-like catalytic fold.
Fruit bromelain is a cysteine endopeptidase from the pineapple plant Ananas comosus, scarcely inhibited by chicken cystatin. It hydrolyzes proteins with broad specificity for peptide bonds; Bz-Phe-Val-Arg-NHMec is a good synthetic substrate, but it shows no action on Z-Arg-Arg-NHMec, unlike stem bromelain. A related enzyme, pinguinain, is obtained from Bromelia pinguin but is inhibited by chicken cystatin. In the fruit it contributes to ripening and protein mobilization. It features a papain-like catalytic fold.
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