Micrococcal nuclease
Definition:
EC Tree > 3 Hydrolases > 3.1 Acting on ester bonds > 3.1.31 Endoribonucleases that are active with either ribo- or deoxyribonucleic acids and produce 3'-phosphomonoesters > 3.1.31.1 micrococcal nuclease
Micrococcal nuclease is a secreted Ca2+-dependent endonuclease from Staphylococcus aureus (Micrococcus pyogenes) that hydrolyses both double- and single-stranded DNA and RNA endonucleolytically, releasing nucleoside 3'-phosphates and 3'-phosphooligonucleotides as end products; it preferentially attacks A/T-rich regions and is widely used to map chromatin accessibility and to digest chromatin into mononucleosomes. It is distributed across Eukaryota and Bacteria and adopts an OB-fold nuclease domain.
Micrococcal nuclease is a secreted Ca2+-dependent endonuclease from Staphylococcus aureus (Micrococcus pyogenes) that hydrolyses both double- and single-stranded DNA and RNA endonucleolytically, releasing nucleoside 3'-phosphates and 3'-phosphooligonucleotides as end products; it preferentially attacks A/T-rich regions and is widely used to map chromatin accessibility and to digest chromatin into mononucleosomes. It is distributed across Eukaryota and Bacteria and adopts an OB-fold nuclease domain.
Biomedical Dictionary
The Biomedical Dictionary is a comprehensive and professional collection of biological academic terms and subject datas. All explanations are supported by authoritative books or high impact factor literatures, and you can acquire accurate explanations of the biomedical terms you want to know.
Molarity Calculator
Dilution Calculator
