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Cat. No.: HY-D3496

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BODIPY 576/589 NHS ester

Name: BODIPY 576/589 NHS ester
Brand: MedChemExpress
SKU: HY-D3496
Availability: PreOrder

(Synonyms: BODIPY 576/589 Succinimidyl ester)

BODIPY 576/589 NHS ester (BODIPY 576/589 Succinimidyl ester) is an active fluorophore containing the BODIPY 576/589 fluorophore and an NHS ester. BODIPY 576/589 NHS ester can covalently couple red fluorescence to biomolecules containing primary amines (-NH2), such as proteins, antibodies, peptides, and oligonucleotides. BODIPY 576/589 NHS ester can also be used to prepare BODIPY 576/589-Met-tRNA_fMet to achieve N-terminal co-translational labeling of nascent proteins (Ex/Em = 576/589 nm).

For research use only. We do not sell to patients.

Formula: C₂₁H₁₉BF₂N₄O₄
Molecular Weight:440.21

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

BODIPY 576/589 NHS ester Chemical Structure

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
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Biological Activity

Description

In Vitro

BODIPY 576/589 NHS ester (0.1 μM; 20 min) enables the creation of a fluorescently labeled initiator tRNA that supports selective N-terminal co-translational labeling and sensitive detection of nascent apoMb in Δtig E. coli cell-free transcription/translation systems^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

Molecular Weight 440.21
Formula C₂₁H₁₉BF₂N₄O₄
SMILES

O=C(ON1C(CCC1=O)=O)CCCC(C=C2)=[N+](C2=C3)[B-](F)(F)N4C3=CC=C4C5=CC=CN5
Synonyms

BODIPY 576/589 Succinimidyl ester
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Dalphin MD, et al. KLR-70: A Novel Cationic Inhibitor of the Bacterial Hsp70 Chaperone. Biochemistry. 2020 May 26;59(20):1946-1960. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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