121714-70-3
Chemical Structure
Biotin-11-UTP-1
- CAS No.: 121714-70-3
- Formula:C28H45N6O18P3S
- Molecular Weight:878.67
InChIKey: PQBJGRCYMNVJTJ-MHQRMSOQSA-N
SMILES: O[C@@H]1[C@H](O)[C@@H](COP(OP(OP(O)(O)=O)(O)=O)(O)=O)O[C@H]1N2C(NC(C(/C=C/CNC(CCCCCNC(CCCC[C@H]3[C@](NC(N4)=O)([H])[C@]4([H])CS3)=O)=O)=C2)=O)=O
Biological Activity: Biotin-11-UTP is a biotinylated uridine triphosphate that can be incorporated into short RNA strands via co-transcription with T7 RNA polymerase, and is used for synthesizing labeling reagents and detection markers. After binding to streptavidin, Biotin-11-UTP generates characteristic downward ionic current peaks during nanopore translocation, which allows the differentiation between modified and unmodified RNA. Biotin-11-UTP enables site-specific labeling of short RNA, and is suitable for single-molecule solid-state nanopore sensing detection. Biotin-11-UTP can serve as a raw material for synthesizing biotinylated single-stranded RNA probes, which is applied to rotavirus nucleic acid detection; it can also label single-stranded RNA probes targeting and immobilizing 28S ribosomal RNA in leukemia cells. Biotin-11-UTP can alter the electrophoretic mobility of transcribed RNA in agarose gels, and can be used to monitor the biotinylation degree of RNA probes[1][2][3].
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Biotin-11-UTP-1 | Biotin-11-UTP is a biotinylated uridine triphosphate that can be incorporated into short RNA strands via co-transcription with T7 RNA polymerase, and is used for synthesizing labeling reagents and detection markers. After binding to streptavidin, Biotin-11-UTP generates characteristic downward ionic current peaks during nanopore translocation, which allows the differentiation between modified and unmodified RNA. Biotin-11-UTP enables site-specific labeling of short RNA, and is suitable for single-molecule solid-state nanopore sensing detection. Biotin-11-UTP can serve as a raw material for synthesizing biotinylated single-stranded RNA probes, which is applied to rotavirus nucleic acid detection; it can also label single-stranded RNA probes targeting and immobilizing 28S ribosomal RNA in leukemia cells. Biotin-11-UTP can alter the electrophoretic mobility of transcribed RNA in agarose gels, and can be used to monitor the biotinylation degree of RNA probes. | |||||||||||||||||||||
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References
- [1]. Mohora I, et al. Programmable RNA Nanostructures Enable Nanopore Detection of Cotranscriptionally Introduced RNA Modifications. Nano letters. 2025 Aug 13;25(32):12184-12192.
- [2]. Bellinzoni R, et al. Rotavirus gene detection with biotinylated single-stranded RNA probes. Molecular and cellular probes. 1989 Sep;3(3):233-44.
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[3]. Bauman J G J, et al. Flow cytometric detection of ribosomal RNA in suspended cells by fluorescent in situ hybridization. Cytometry, 1988, 9(6): 517‑524.
