124763-51-5
Chemical Structure
Bis-Tris hydrochloride
- CAS No.: 124763-51-5
- Formula:C8H20ClNO5
- Molecular Weight:245.70
IUPAC Name: 2-(bis(2-hydroxyethyl)amino)-2-(hydroxymethyl)propane-1,3-diol hydrochloride
InChIKey: VEYRVLHAMHQVTC-UHFFFAOYSA-N
SMILES: OCCN(C(CO)(CO)CO)CCO.Cl
Biological Activity: Bis-Tris hydrochloride is an amine buffer suitable for protein and nucleic acid systems with a pH buffer range of 5.8-7.2. Bis-Tris hydrochloride can also be mixed with HEPES and cacodylic acid buffer to create a pH 8 environment and monitor fluorescence emission intensity at 305 nm. Gels formulated with Bis-Tris also avoid protein degradation in samples prepared at higher pH (pH 8.5)[1][2][3].
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Bis-Tris hydrochloride | 99.60% | Bis-Tris hydrochloride is an amine buffer suitable for protein and nucleic acid systems with a pH buffer range of 5.8-7.2. Bis-Tris hydrochloride can also be mixed with HEPES and cacodylic acid buffer to create a pH 8 environment and monitor fluorescence emission intensity at 305 nm. Gels formulated with Bis-Tris also avoid protein degradation in samples prepared at higher pH (pH 8.5). | ||||||||||||||||||||
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References
- [1]. Oh, Soon Jin, et al. Structure and phosphodiesterase activity of Bis-Tris coordinated lanthanide(III) complexes.
- [2]. Williams AS, et al. Domain 4 of the anthrax protective antigen maintains structure and binding to the host receptor CMG2 at low pH. Protein Sci. 2009 Nov;18(11):2277-86. [Content Brief]
- [3]. Hachmann JP, et al. Models of protein modification in Tris-glycine and neutral pH Bis-Tris gels during electrophoresis: effect of gel pH. Anal Biochem. 2005 Jul 15;342(2):237-45. [Content Brief]
