1811539-32-8
Chemical Structure
Janelia Fluor® 549, SE
Synonym(s): JF549, SE; JF549, NHS
- CAS No.: 1811539-32-8
- Formula:C31H25N3O7
- Molecular Weight:551.55
IUPAC Name: 2-(3,6-di(azetidin-1-yl)xanthylium-9-yl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)benzoate
InChIKey: DWUIXAGWHCMXHN-UHFFFAOYSA-N
SMILES: O=C(C1=CC=C(C([O-])=O)C(C2=C3C=CC(N4CCC4)=CC3=[O+]C5=C2C=CC(N6CCC6)=C5)=C1)ON7C(CCC7=O)=O
Biological Activity: Janelia Fluor? 549, SE (JF549, SE) is a cell-permeable fluorescent dye with an NHS ester (succinimidyl ester (SE)) reactive group. NHS esters react with primary amines and are commonly used to conjugate dyes to proteins, antibodies, amine-modified oligonucleotides, and other molecules. Janelia Fluor fluorescent dyes can be chemically conjugated to the specific HaloTag substrate (a chloroalkane ligand) to form a fluorescent ligand, rather than binding directly to the HaloTag protein itself. Janelia Fluor? 549, SE also acts as a ligand for self-labeling tags such as HaloTag and SNAP-tag, and can be used in live-cell imaging studies (Ex/Em = 549 nm/571 nm)[1].
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Janelia Fluor® 549, SE | Janelia Fluor? 549, SE (JF549, SE) is a cell-permeable fluorescent dye with an NHS ester (succinimidyl ester (SE)) reactive group. NHS esters react with primary amines and are commonly used to conjugate dyes to proteins, antibodies, amine-modified oligonucleotides, and other molecules. Janelia Fluor fluorescent dyes can be chemically conjugated to the specific HaloTag substrate (a chloroalkane ligand) to form a fluorescent ligand, rather than binding directly to the HaloTag protein itself. Janelia Fluor? 549, SE also acts as a ligand for self-labeling tags such as HaloTag and SNAP-tag, and can be used in live-cell imaging studies (Ex/Em = 549 nm/571 nm). | |||||||||||||||||||||
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