3100316-45-5
Chemical Structure
T-sCPY-s500R-H
- CAS No.: 3100316-45-5
- Formula:C86H98ClF6N13O15S2
- Molecular Weight:1767.35
InChIKey: UYNKWGSARIRQDY-UHFFFAOYSA-N
SMILES: NC1=NC(N)=C(CC2=CC(OC)=C(OCCCCNC(C3=CC(C4(C(C=CC(N(C)C)=C5)=C5C(C)(C)C6=C4C=CC(N(C)C)=C6)N(S(=O)(CCNC(CCCS(=O)(N7C8(C(C=CC(NCC(F)(F)F)=C9)=C9OC%10=C8C=CC(NCC(F)(F)F)=C%10)C(C=C(C(NCCOCCOCCCCCCCl)=O)C=C%11)=C%11C7=O)=O)=O)=O)C%12=O)=C%12C=C3)=O)C(OC)=C2)C=N1
Biological Activity: T-sCPY-s500R-H is a NADPH-based FRET ratiometric biosensor probe. T-sCPY-s500R-H forms a 1:1 FRET pair upon binding to the eDHFR-HaloTag fusion protein through conjugation of the donor s500R (green fluorescence) and the acceptor sCPY (red fluorescence) with the HaloTag ligand and the eDHFR ligand (TMP), respectively; NADPH binding to eDHFR induces a conformational change that converts sCPY from a colorless spirolactam form to a red fluorescent form, enabling quantification of NADPH levels through the ratio of red/green fluorescence intensity (Ex = 488 or 490 nm; green channel Em = 500-580 nm; red channel Em = 600-700 nm). T-sCPY-s500R-H is applicable to research in fields such as cell metabolism studies, oxidative stress and NADPH homeostasis analysis, tumor metabolism, and NADPH-modulating drug screening[1].
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T-sCPY-s500R-H | T-sCPY-s500R-H is a NADPH-based FRET ratiometric biosensor probe. T-sCPY-s500R-H forms a 1:1 FRET pair upon binding to the eDHFR-HaloTag fusion protein through conjugation of the donor s500R (green fluorescence) and the acceptor sCPY (red fluorescence) with the HaloTag ligand and the eDHFR ligand (TMP), respectively; NADPH binding to eDHFR induces a conformational change that converts sCPY from a colorless spirolactam form to a red fluorescent form, enabling quantification of NADPH levels through the ratio of red/green fluorescence intensity (Ex = 488 or 490 nm; green channel Em = 500-580 nm; red channel Em = 600-700 nm). T-sCPY-s500R-H is applicable to research in fields such as cell metabolism studies, oxidative stress and NADPH homeostasis analysis, tumor metabolism, and NADPH-modulating drug screening. | |||||||||||||||||||||
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