460085-45-4
Chemical Structure
4-MU-α-GlcNS sodium
- CAS No.: 460085-45-4
- Formula:C16H18NNaO10S
- Molecular Weight:439.37
IUPAC Name: ((2R,3R,4S,5R,6S)-3,4,5-trihydroxy-6-((4-methyl-2-oxo-2H-chromen-7-yl)oxy)tetrahydro-2H-pyran-2-yl)methyl hydrogen sulfate
InChIKey: LMECUNAMBHBGFU-MYDYNYEJSA-M
SMILES: O=S(N[C@H]([C@H]([C@@H]([C@H](O1)CO)O)O)[C@H]1OC2=CC=C(C(C)=CC(O3)=O)C3=C2)(O[Na])=O
Biological Activity: 4-MU-α-GlcNS sodium is a fluorogenic substrate for heparan sulfate sulfamidase, with apparent Km values of 4 mM and 8 mM for fibroblast and leukocyte enzymes, respectively. 4-MU-α-GlcNS sodium undergoes enzymatic desulfation to generate an intermediate, which requires a second incubation with yeast α-glucosidase to hydrolyze and release 4-methylumbelliferone, detected by fluorescence at Ex/Em = 365/450 nm. 4-MU-α-GlcNS sodium is suitable for fibroblast homogenates, leukocytes, and dried leukocyte filter paper samples, and can be used for the detection and study of enzyme activity in mucopolysaccharidosis type IIIA[1][2].
| Cat. No. | Product Name | Purity | Description | Pricing | |||||||||||||||||||
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4-MU-α-GlcNS sodium | 99.82% | 4-MU-α-GlcNS sodium is a fluorogenic substrate for heparan sulfate sulfamidase, with apparent Km values of 4 mM and 8 mM for fibroblast and leukocyte enzymes, respectively. 4-MU-α-GlcNS sodium undergoes enzymatic desulfation to generate an intermediate, which requires a second incubation with yeast α-glucosidase to hydrolyze and release 4-methylumbelliferone, detected by fluorescence at Ex/Em = 365/450 nm. 4-MU-α-GlcNS sodium is suitable for fibroblast homogenates, leukocytes, and dried leukocyte filter paper samples, and can be used for the detection and study of enzyme activity in mucopolysaccharidosis type IIIA. | ||||||||||||||||||||
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References
- [1]. Karpova EA, et al. A fluorimetric enzyme assay for the diagnosis of Sanfilippo disease type A (MPS IIIA). Journal of inherited metabolic disease. 1996;19(3):278-85. [Content Brief]
- [2]. Civallero G, et al. Assay of heparan-N-sulfamidase in dried leukocytes impregnated in filter paper: a new tool for the identification of mucopolisaccharidosis IIIA and potentially other lysosomal disorders. Molecular genetics and metabolism. 2013 Apr;108(4):267-8.
