959755-19-2
Chemical Structure
3'-O-(2-Nitrobenzyl)-dCTP
- CAS No.: 959755-19-2
- Formula:C16H21N4O15P3
- Molecular Weight:602.28
InChIKey: ZSMGSQOHORZZNZ-GZBFAFLISA-N
SMILES: OP(OC[C@H]1O[C@@H](N2C(N=C(N)C=C2)=O)C[C@@H]1OCC3=CC=CC=C3[N+]([O-])=O)(OP(OP(O)(O)=O)(O)=O)=O
Biological Activity: 3'-O-(2-Nitrobenzyl)-dCTP is a reversible terminator. 3'-O-(2-Nitrobenzyl)-dCTP can be recognized and incorporated by polymerases, thereby temporarily terminating primer extension during DNA synthesis. After incorporation into the extending DNA strand, 3'-O-(2-Nitrobenzyl)-dCTP temporarily halts the DNA polymerase extension reaction, and its 3'-O-(2-nitrobenzyl) blocking group can be removed by laser irradiation to regenerate a free 3'-OH for subsequent primer extension cycles. 3'-O-(2-Nitrobenzyl)-dCTP is applicable for resolving homopolymeric regions in DNA templates[1].
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3'-O-(2-Nitrobenzyl)-dCTP | 3'-O-(2-Nitrobenzyl)-dCTP is a reversible terminator. 3'-O-(2-Nitrobenzyl)-dCTP can be recognized and incorporated by polymerases, thereby temporarily terminating primer extension during DNA synthesis. After incorporation into the extending DNA strand, 3'-O-(2-Nitrobenzyl)-dCTP temporarily halts the DNA polymerase extension reaction, and its 3'-O-(2-nitrobenzyl) blocking group can be removed by laser irradiation to regenerate a free 3'-OH for subsequent primer extension cycles. 3'-O-(2-Nitrobenzyl)-dCTP is applicable for resolving homopolymeric regions in DNA templates. | |||||||||||||||||||||
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