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Cat. No.: HY-E70445

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Chondroitinase AC II

Name: Chondroitinase AC II
Brand: MedChemExpress
SKU: HY-E70445
Price: 1681.00 USD
Availability: InStock
Rating: 5 (1 reviews)

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Chondroitinase AC II is a glycosaminoglycan lyase (EC 4.2.2.5) discovered from Arthrobacter sp. CS01. Chondroitinase AC II exhibits broad substrate specificity toward CS-A, CS-C, and HA. Chondroitinase AC II cleaves the β-1,4-glycosidic bond between D-glucuronic acid and N-acetylgalactosamine through a β-elimination mechanism, producing unsaturated disaccharides and thereby degrading chondroitin sulfate and hyaluronic acid. Chondroitinase AC II activity is enhanced by Mn²⁺ and inhibited by Zn²⁺ and Hg²⁺. Chondroitinase AC II is used for CS oligosaccharide preparation and research related to nerve injury.

For research use only. We do not sell to patients.

CAS No.: 9047-57-8

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Please store the product under the recommended conditions in the Certificate of Analysis.

Chondroitinase AC II Chemical Structure

CAS No.: 9047-57-8

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
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- In Vitro
Chemical Information
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Biological Activity

Description

In Vitro

Carbohydrate Metabolism

ChSase AC II (0.03125-2 mg/mL) exhibits broad substrate specificity, with V_max values of 186, 168, and 472 µM/min and K_m values of 2.44, 1.68, and 0.165 µM for CS-A, CS-C, and HA, respectively^[1].
ChSase AC II (6 h) activity is enhanced by Mn²⁺ and strongly inhibited by Hg²⁺^[1].
ChSase AC II (1 μg purified enzyme; 0-5 h) exhibits an exolytic mode of action, completely hydrolyzing CS-A, CS-C, and HA into low-polymerization-degree oligosaccharides^[1].
The V_max of ChSase AC II for the substrate CSC is 0.236 μmol/min/mL, and the K_m is 0.483 mg/mL^[2].
ChSase AC II (25-50 °C; 30-180 min) retains 32.1% relative activity after incubation at 40 °C for 120 min^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Related Protocols :

Carbohydrates and Mucins: Alcian Blue/Alcian Blue-PAS Staining

Chemical Information

CAS No. 9047-57-8
Appearance Liquid
Color Colorless to light yellow
SMILES

[Chondroitinase AC II]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (270 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Fang Y, et al. Expression, Purification and Characterization of Chondroitinase AC II from Marine Bacterium sp. CS01. Marine drugs. 2019 Mar 20;17(3):185.

[2]. Fang Y, et al. Expression, purification and characterisation of chondroitinase AC II with glyceraldehyde-3-phosphate dehydrogenase tag and chaperone (GroEs-GroEL) from Arthrobacter sp. CS01. International journal of biological macromolecules. 2019 May 15;129:471-476.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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