CP61 acetate
Based on 1 Customer Validation
CP61 acetate is a CtBP1 and CtBP2 inhibitor that binds to dimeric CtBP1 and allosterically inhibits NADH binding, disrupting the NADH-dependent dimerization of CtBP1 and CtBP2, inducing aberrant mitosis and micronucleus formation, thereby inhibiting cell proliferation and colony formation. CP61 acetate can be used in breast cancer research.
For research use only. We do not sell to patients.
- Purity : 98.92%
- Formula: C50H73N13O12S·xC2H4O2
- Molecular Weight:1080.26 (free base)
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
CtBP1 |
CtBP2 |
In Vitro
CP61 (50 μM) acetate selectively increases abnormal mitosis and prolongs mitotic duration in highly glycolytic MCF-7 cells, but has no such effect in MDA-MB-453 cells with lower glycolysis[1].
CP61 acetate inhibits CtBP2 homodimerization with an IC50 of 19 μM in ELISA assays[1].
CP61 acetate disrupts CtBP1 dimerization in a dose-dependent manner[1].
CP61 acetate inhibits NADH binding to CtBP1 through an allosteric mechanism and binds to dimeric CtBP1 with an affinity of 3-11 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MCF-7
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Concentration:50 μM
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Incubation Time:48 h
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Result:Did not affect the protein abundance of CtBP1 or CtBP2 in MCF-7 cells when treated with CP61-TAT.
- BrdU Incorporation Assay
- Somatic Cell Culture
- CFSE Dye Dilution Proliferation Assay
- Dye-dilution cell tracking and proliferation staining
- CCK-8/WST-8 Cell Proliferation Assay
- Protocol for Cell Cycle
- Cell Counting-Based Growth Curve Assay
- Colony Formation (Clonogenic) Assay
- Breast Cancer Modeling
- Cell Viability Determination by MTT Colorimetric Assay
- EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
- Ki-67 Immunostaining Proliferation Assay
- PCNA Immunodetection Proliferation Assay
- Protocol for Cell Counting and Cell Density Analysis
- Soft Agar Colony Formation Assay
- MTT Cell Proliferation Assay
- Genotoxicity/Mutagenicity Study
Chemical Information
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Appearance Solid
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Molecular Weight 1080.26 (free base)
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Formula C50H73N13O12S·xC2H4O2
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SMILES
O=C(NCC(N[C@@H](CC1=CNC2=CC=CC=C12)C(N[C@@H]([C@H](O)C)C(N[C@@H](C(C)C)C(N[C@@H](C(C)C)C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CCSC)C(N[C@H]3CC4=CC=C(C=C4)O)=O)=O)=O)=O)=O)=O)=O)[C@H](CO)NC3=O.CC(O)=O.[x]
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Sequence
cyclo(Ser-Gly-Trp-Thr-Val-Val-Arg-Met-Tyr)
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Sequence Shortening
cyclo(SGWTVVRMY)
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Purity & Documentation
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Data Sheet (300 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
CP61 acetate Related Classifications
HY-P10812A Related Classifications
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