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Cat. No.: HY-128890A

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DOTA-NHS-ester hexafluorophosphate TFA

Name: DOTA-NHS-ester hexafluorophosphate TFA
Brand: MedChemExpress
SKU: HY-128890A
Availability: PreOrder

2 Cited Publications

Customer Review

Based on 2 publication(s) in Google Scholar

DOTA-NHS-ester hexafluorophosphate TFA is a bifunctional reagent containing a DOTA chelating group and an NHS ester. DOTA-NHS-ester hexafluorophosphate TFA can be conjugated to proteins/antibodies/HSA via primary amino groups for radiolabeling with radiometals, and when combined with 16-amino-1-hexadecanethiol hydrochloride, it enables the construction of self-assembled monolayers on gold surfaces for lectin microarrays. DOTA-NHS-ester hexafluorophosphate TFA can be used to prepare DOTA-HSA-Z (HER2:342) imaging probes, as well as to construct Trop2-targeted radiotherapeutic and diagnostic probes for pancreatic cancer.

For research use only. We do not sell to patients.

CAS No.: 1823122-52-6
Formula: C₂₀H₃₁N₅O₁₀_.C₂HF₃O₂_.F₆P_.H
Molecular Weight:761.48

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (277 KB)

DOTA-NHS-ester hexafluorophosphate TFA Chemical Structure

CAS No.: 1823122-52-6

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
Publications (2)
Purity & Documentation
References
Calculators
Help & FAQs
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Biological Activity

Description

In Vitro

DOTA-NHS ester (3.78 mg; 2 h) hexafluorophosphate TFA successfully conjugates to HSA, yielding a DOTA-HSA bioconjugate with approximately two DOTA molecules per HSA molecule^[1].
DOTA-NHS-ester (10 mM; overnight) hexafluorophosphate TFA in combination with 16-amino-1-hexadecanethiol hydrochloride successfully modifies gold chip surfaces, producing a rough surface topography and characteristic infrared absorption peaks consistent with covalent coupling of the reagent^[2].
DOTA-NHS-ester (5-20 mM; 2 h) hexafluorophosphate TFA modification concentration on gold biochips positively correlates with IgG protein binding capacity, with 10 mM selected as the optimal working concentration balancing performance and reagent efficiency^[2].
DOTA-NHS-ester (10 mM; overnight) hexafluorophosphate TFA modification of gold biochips proceeds effectively at both 25 °C and 37 °C, with 25 °C selected as the optimal working temperature for practical convenience^[2].
DOTA-NHS-ester (10 mM; overnight) hexafluorophosphate TFA modified gold biochips support specific, concentration-dependent binding of ConA lectin and its corresponding antibody, validating the platform's suitability for lectin-based assays^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 1823122-52-6
Molecular Weight 761.48
Formula C₂₀H₃₁N₅O₁₀_.C₂HF₃O₂_.F₆P_.H
SMILES

O=C(CN1CCN(CCN(CCN(CC1)CC(O)=O)CC(ON2C(CCC2=O)=O)=O)CC(O)=O)O.O=C(C(F)(F)F)O.[F-][P+5]([F-])([F-])([F-])([F-])[F-].[H+]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (2)

All In Vivo Efficacy Study

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Journal Impact Factor

Cell Stem Cell

2026 Sep 3;33(9):1444-1458.e9. PMID: 42624106
Adv Nanobiomed Res

2023 Sep 3.

DOTA-NHS-ester hexafluorophosphate TFA purchased from MedChemExpress. Usage Cited in: Adv Nanobiomed Res. 2023 Sep 3.

PSP–NH2 was incubated with DOTA–NHS–ester for 1 h and conjugated with mPEG–NHS. Then, the particles were mixed with ⁶⁴Cu (OAc)₂ at 50 ℃ for 1 h to obtain the ⁶⁴Cu-labelled PSP. PSP@Ab@PEG exhibited increased blood circulation in BALB/c mice with blood clearance half-life periods found to be 12 min.

DOTA-NHS-ester hexafluorophosphate TFA purchased from MedChemExpress. Usage Cited in: Adv Nanobiomed Res. 2023 Sep 3.

PSP–NH2 was incubated with DOTA–NHS–ester for 1 h and conjugated with mPEG–NHS. Then, the particles were mixed with ⁶⁴Cu (OAc)₂ at 50 ℃ for 1 h to obtain the ⁶⁴Cu-labelled PSP. More PSP–NH2 accumulated in the liver site while a lot more PSP@Ab@PEG accumulated in the spleen.

Purity & Documentation

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Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Hoppmann S, et al. Radiolabeled affibody-albumin bioconjugates for HER2-positive cancer targeting. Bioconjugate chemistry. 2011 Mar 16;22(3):413-21. [Content Brief]

[2]. Gao Y, et al. Establishment of a 1, 4, 7, 10-tetraazacyclododecane-1,4,7,10-tetraacetic acid mono-N-hydroxysuccinimide ester (DOTA-NHS-ester) based lectin microarray for efficiently detecting serum glycans in gastric cancers. Analytical biochemistry. 2020 May 15;597:113686.

[3]. Li C, et al. Theranostic application of Cu/Lu-labeled anti-Trop2 monoclonal antibody in pancreatic cancer tumor models. European journal of nuclear medicine and molecular imaging. 2022 Dec;50(1):168-183. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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DOTA-NHS-ester hexafluorophosphate TFA

2 Cited Publications

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Biological Activity
- Description
- In Vitro
Chemical Information
Publications (2)
Purity & Documentation
References

1/16
1/16

DOTA-NHS-ester hexafluorophosphate TFA purchased from MedChemExpress. Usage Cited in: Adv Nanobiomed Res. 2023 Sep 3.

Customer Validation
PSP–NH2 was incubated with DOTA–NHS–ester for 1 h and conjugated with mPEG–NHS. Then, the particles were mixed with ⁶⁴Cu (OAc)₂ at 50 ℃ for 1 h to obtain the ⁶⁴Cu-labelled PSP. PSP@Ab@PEG exhibited increased blood circulation in BALB/c mice with blood clearance half-life periods found to be 12 min.
1/16

DOTA-NHS-ester hexafluorophosphate TFA purchased from MedChemExpress. Usage Cited in: Adv Nanobiomed Res. 2023 Sep 3.

Customer Validation
PSP–NH2 was incubated with DOTA–NHS–ester for 1 h and conjugated with mPEG–NHS. Then, the particles were mixed with ⁶⁴Cu (OAc)₂ at 50 ℃ for 1 h to obtain the ⁶⁴Cu-labelled PSP. More PSP–NH2 accumulated in the liver site while a lot more PSP@Ab@PEG accumulated in the spleen.

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DOTA-NHS-ester hexafluorophosphate TFA

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