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Cat. No.: HY-D3407

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Dusquench2 dT phosphoramidite

Dusquench2 dT phosphoramidite is a non-fluorescent dark quencher that absorbs emission from nearby fluorophores without generating its own fluorescent signal. Featuring a dT phosphoramidite moiety, it is designed for incorporation during oligonucleotide synthesis. Dusquench2 dT phosphoramidite is primarily used in FRET-based nucleic acid probes, molecular beacons, and multiplex qPCR assays.

For research use only. We do not sell to patients.

Formula: C₇₅H₈₉N₁₂O₁₄P
Molecular Weight:1413.55

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Dusquench2 dT phosphoramidite Chemical Structure

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
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Biological Activity

Description

In Vitro

Dusquench2 NHS ester can be used in combination with dyes emitting yellow, orange to red light (such as Cyanine3, Cyanine5, ROX, Texas Red, AF 555, AF 594 and AF 647).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Related Protocols :

Real Time qPCR (Q-PCR)

Chemical Information

Molecular Weight 1413.55
Formula C₇₅H₈₉N₁₂O₁₄P
SMILES

COC1=CC(/N=N/C2=CC=C(N(CCCCCC(NCCCCCC(NCC#CC3=CN([C@H]4O[C@@H](COC(C5=CC=C(C=C5)OC)(C6=CC=CC=C6)C7=CC=C(C=C7)OC)[C@H](OP(N(C(C)C)C(C)C)OCCC#N)C4)C(NC3=O)=O)=O)=O)C)C=C2)=C(OC)C=C1/N=N\C8=CC=C([N+]([O-])=O)C=C8
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Torimura M, et al. Fluorescence-quenching phenomenon by photoinduced electron transfer between a fluorescent dye and a nucleotide base. Anal Sci. 2001;17(1):155-160. [Content Brief]

[2]. Lakowicz J R. Quenching of fluorescence[M]//Principles of fluorescence spectroscopy. Boston, MA: Springer US, 1983: 257-301.

Calculators

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

Minimum order quantity

100 mg

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