EPO mimetic peptide-1
Based on 1 Customer Validation
EPO mimetic peptide-1 (EMP-1), a 20 amino acid peptide. EPO mimetic peptide-1 stimulates cell proliferation, affects cell cycle and induces the production of reticulocytes in vivo.
For research use only. We do not sell to patients.
- Purity : 98.92%
- CAS No.: 180088-82-8
- Formula: C94H134N26O25S2
- Molecular Weight:2092.36
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
EPO mimetic peptide-1 shows inhibition of tracer 125I-labeled EPO binding with an IC50 value of 0.2 μM[1]. EPO mimetic peptide-1 (0-50 mM; 48 h) stimulates proliferation of both TF-1 and B6Sut cells in a dose-dependent manner[1]. EPO mimetic peptide-1 (1 and 10 μM) promots erythroid colony formation from human peripheral blood and murine bone marrow[1]. EPO mimetic peptide-1 (10 μM; 10 min) induces the phosphorylation of proteins[1]. EPO mimetic peptide-1 (1 and 10 μM; 0-18 h) affects cell cycle[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:FDCP-l/mEPOR cell line
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Concentration:10 μM
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Incubation Time:10 min
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Result:Induced the phosphorylation of proteins with apparent molecular weights of 140, 95, 70, and 55 Kd.
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Cell Line:FDCP-l/mEPOR cell line
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Concentration:10 μM
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Incubation Time:0, 6, 8, 10, 12 and 18 h
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Result:Increased cell progression into S phase.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
- BrdU Incorporation Assay
- Somatic Cell Culture
- CFSE Dye Dilution Proliferation Assay
- Dye-dilution cell tracking and proliferation staining
- CCK-8/WST-8 Cell Proliferation Assay
- Protocol for Cell Cycle
- Cell Counting-Based Growth Curve Assay
- Colony Formation (Clonogenic) Assay
- Cell Viability Determination by MTT Colorimetric Assay
- EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
- Ki-67 Immunostaining Proliferation Assay
- PCNA Immunodetection Proliferation Assay
- Flow cytometric DNA-content cell-cycle staining
- Protocol for Cell Counting and Cell Density Analysis
- MTT Cell Proliferation Assay
Chemical Information
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CAS No. 180088-82-8
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Appearance Solid
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Molecular Weight 2092.36
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Formula C94H134N26O25S2
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Synonyms
EMP-1
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Sequence
Gly-Gly-Thr-Tyr-Ser-Cys-His-Phe-Gly-Pro-Leu-Thr-Trp-Val-Cys-Lys-Pro-Gln-Gly-Gly-NH2 (disulfide bridge:Cys6-Cys15)
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Sequence Shortening
GGTYSCHFGPLTWVCKPQGG-NH2 (disulfide bridge:Cys6-Cys15)
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
DMSO : 25 mg/mL (11.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.4779 mL | 2.3896 mL | 4.7793 mL | 11.9482 mL |
| 5 mM | 0.0956 mL | 0.4779 mL | 0.9559 mL | 2.3896 mL | |
| 10 mM | 0.0478 mL | 0.2390 mL | 0.4779 mL | 1.1948 mL |
EPO mimetic peptide-1 Related Classifications
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