HDAC-IN-92
HDAC-IN-92 is a pan-HDAC inhibitor with an IC50 of 12.58 µM in A2780 cells. HDAC-IN-92 demonstrates broad-spectrum, notable cytotoxic activity against a range of human cancer cell lines, including ovarian, liver, and breast carcinomas. HDAC-IN-92 causes apoptosis and demonstrates a notable decrease in tumor cell colony formation. HDAC-IN-92 inhibits the formation of blood vessels in the chick chorioallantoic membrane (CAM). HDAC-IN-92 exhibits anti-tumor effect in a 4T1 tumor-bearing mouse model. HDAC-IN-92 can be used for research targeting solid tumor.
For research use only. We do not sell to patients.
- Formula: C19H17FN4O2S
- Molecular Weight:384.43
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
HDAC-IN-92 (compound 6g) (18 h) demonstrates significant cytotoxicity in a broad panel of cancer cell lines, with IC50s of 22.17 μM (MCF-7), 8.46 μM (HepG2), 8.10 μM (A2780), 26.89 μM (A2780cis), 75.90 μM (HT29), and 14.11 μM (4T1)[1].
HDAC-IN-92 (18 h) demonstrates a correlation between its cytotoxicity against cancer cells and its pan-HDAC inhibitory activity, suggesting that its antitumor effect may be mediated by the inhibition of histone deacetylases[1].
HDAC-IN-92 (8.10 μM, 24 h) significantly promotes apoptosis, increasing the total apoptotic population from 1.82 % to 22.17 %, and inhibits cell migration in A2780 cells[1].
HDAC-IN-92 (22.17 μM, 10 days) remarkably reduces colony formation in MCF-7 cancer cells[1].
HDAC-IN-92 (5 μM, 96 h) inhibits CAM angiogenesis as effectively as Vorinostat (HY-10221), as evidenced by a marked reduction in branching points, tube number, and total tube length[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:A2780 cells
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Concentration:8.10 nM
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Incubation Time:24 h
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Result:Increased percentage early and late apoptosis cells from 1.82 % of the control to 22.17 %.
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Cell Line:MCF-7 cells
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Concentration:22.17 nM
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Incubation Time:24 h
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Result:Reduces colony formation in MCF-7 cancer cells.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Female Balb/c mice (4-6 weeks old) injected subcutaneously with 4T1 cells[1]
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Dosage:50 mg/kg
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Administration:i.p., every other day for 21 days
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Result:Reduced tumor volume better than Vorinostat.
Exerted no significant effects on the body weight or survival rates of mice.
Induced tumor cell apoptosis without exhibiting toxicity in major organs.
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Cell Cytotoxicity Assay
- Annexin V plus membrane-impermeant dye apoptosis staining
- Apoptosis Solutions
- Soft Agar Colony Formation Assay
- Colony Formation (Clonogenic) Assay
- Cell Viability Determination by MTT Colorimetric Assay
- How to Choose the Right Model Animal
Chemical Information
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Molecular Weight 384.43
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Formula C19H17FN4O2S
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SMILES
O=C(NO)C1=CC=C(/N=C/C2=CN=C(SCC3=CC=CC(F)=C3)N2C)C=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
HDAC-IN-92 Related Classifications
HY-175857 Related Classifications
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