Herbicide safener-6

Herbicide safener-6 (compound I-3) (10-20 mg/kg; 12 h seed soaking, followed by cultivation to the two-leaf stage and herbicide application) significantly increases HPPD activity in living tissues of rice roots and aboveground parts (leaves), raising the activity to 60.15 U/L and 68.17 U/L, respectively. It significantly stimulates endogenous GSH biosynthesis and increases GSH content, elevating the levels to 1.48 μmol/g and 7.76 μmol/g, respectively. It substantially enhances glutathione-S-transferase (GST) activity, increasing the activity to 71.88 nmol/min/mL and 69.25 nmol/min/mL, respectively. It significantly raises CYP450 activity, boosting the activity to 141.34 pmol/L and 90.58 pmol/L, respectively. It greatly increases peroxidase (POD) activity, elevating the activity to 29025.00 U/g and 22765.45 U/g, respectively. It alleviates herbicide-induced oxidative stress, thereby reducing the compensatorily elevated superoxide dismutase (SOD) activity to 140.15 U/g and 218.28 U/g, respectively^[1].

Herbicide safener-6 has a structure and physicochemical properties similar to those of benzobicyclon, including an ALog p value of 3.99 and 4 rotatable bonds, which supports its competitive binding to HPPD^[1].
Herbicide safener-6 competitively binds to the active site of (PDB ID: 5YWG) with Benzobicyclon, exhibits a CDOCKER energy of 14.02 kcal mol⁻¹, interacts with key residues, and occupies the binding pocket^[1].