ICR-191 dihydrochloride
ICR-191 dihydrochloride enhances Cisplatin (HY-17394)- DNA binding and sensitizes cells to cisplatin. ICR-191 dihydrochloride induces a significant increase in the expression of phosphorylated H2AX (γH2AX), particularly in DNA-replicating cells. ICR-191 dihydrochloride can be used for the study of leukemia.
For research use only. We do not sell to patients.
- CAS No.: 17070-45-0
- Formula: C19H23Cl4N3O
- Molecular Weight:451.22
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
All DNA/RNA Synthesis Isoforms
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Biological Activity
Description
In Vitro
ICR-191 dihydrochloride (16 h)-induced mutations (such as G insertion) create new GG sites in CHO-ICR cells, leading to an increase in the number of cisplatin binding sites. The IC50 of cisplatin in CHO-ICR cells (IC50 = 15.96 μM) is reduced to half that of CHO wild-type (WT) cells (IC50 = 25.42 μM)[1].
ICR-191 dihydrochloride (8-12 μM) fluorescence is quenched with increasing CHL concentration[2].
ICR-191 dihydrochloride (8-10.1 μM) binds to DNA via intercalation, but its affinity is relatively low[2].
ICR-191 dihydrochloride (1.2 μM, 1 h) can induce histone H2AX phosphorylation (γH2AX) in HL-60 cells, especially in S-phase cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 17070-45-0
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Appearance Solid
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Molecular Weight 451.22
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Formula C19H23Cl4N3O
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Color Light yellow to green yellow
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SMILES
ClC1=CC2=NC(C=CC(OC)=C3)=C3C(NCCCNCCCl)=C2C=C1.Cl.Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
DMSO : 1.67 mg/mL (3.70 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (644 KB)
- English - EN (644 KB)
- Français - FR (644 KB)
- Deutsch - DE (644 KB)
- Norwegian - NO (644 KB)
- Español - ES (644 KB)
- Swedish - SV (644 KB)
- Italian - IT (644 KB)
- Korean - KR (644 KB)
- Portuguese - PT (644 KB)
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Handling Instructions (2659 KB)
References
[1]. Shao C, et al. Pt-seq unveils the genomic binding pattern of platinum-based drugs. Sci Adv. 2025 Oct 31;11(44):eadx6809. [Content Brief]
[2]. Pietrzak M, et al. Attenuation of acridine mutagen ICR-191--DNA interactions and DNA damage by the mutagen interceptor chlorophyllin. Biophys Chem. 2008 Jun;135(1-3):69-75. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2162 mL | 11.0811 mL | 22.1621 mL | 55.4053 mL |
ICR-191 dihydrochloride Related Classifications
HY-W414334 Related Classifications
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