IR-775 chloride
Based on 1 Customer Validation
IR-775 chloride is a cyanine dye with near-infrared absorption (Ex/Em = 775/795 nm). IR-775 chloride acts as a photosensitizer or photothermal agent, and induces apoptosis via generating ROS or exerting photothermal effects upon irradiation; its combination with 2-methoxyestradiol (HY-12033) inhibits SOD2 to enhance photodynamic therapy (PDT) efficacy, and its liposomal encapsulation enables phytotherapy-photothermal therapy (PTT) synergy. IR-775 chloride is applicable for investigating PDT/PTT and near-infrared fluorescence imaging in ovarian cancer and breast cancer.
For research use only. We do not sell to patients.
- CAS No.: 199444-11-6
- Formula: C32H36Cl2N2
- Molecular Weight:519.55
-
Storage:
Store at room temperature, keep dry and cool.
In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Protocols
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Description
In Vitro
IR-775 (2-10 μM; 24-72 h) chloride is a potent photosensitizer in proliferative diabetic retinopathy (PDR). It reduces the viability of human ovarian adenocarcinoma cell line (SKOV-3) and human breast adenocarcinoma cell line (MDA-MB-231) in a time-dependent manner, and exhibits stronger cytotoxicity when combined with 2-methoxyestradiol plus irradiation[1].
IR-775 (6 μM; 2-24 h) chloride accumulates in non-nuclear organelles of human ovarian adenocarcinoma cell line (SKOV-3) and human breast adenocarcinoma cell line (MDA MB-231), and an increase in uptake is observed during the 2 h to 24 h incubation period[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:human ovarian adenocarcinoma (SKOV-3), human breast adenocarcinoma (MDA MB-231)
-
Concentration:2 μM; 4 μM; 6 μM; 8 μM; 10 μM
-
Incubation Time:24 h (pre-incubation); 24 h (post-irradiation incubation); 72 h (post-irradiation incubation)
-
Result:Reduced viability in both cell lines when treated with 2-10 μM for 24 h.
Identified 6 μM as the non-toxic dose.
Reduced MDA MB-231 viability to ~80% and SKOV-3 viability to ~88% after 24 h post-irradiation incubation when used alone in photodynamic reaction (PDR).
Reduced MDA MB-231 viability to 53% after 24 h and 33% after 72 h post-irradiation incubation, and SKOV-3 viability to 60% after 24 h and 45% after 72 h post-irradiation incubation when combined with 2-methoxyestradiol and irradiation.
Showed minimal cytotoxicity alone without irradiation.
-
Cell Line:human ovarian adenocarcinoma (SKOV-3), human breast adenocarcinoma (MDA MB-231)
-
Concentration:6 μM
-
Incubation Time:24 h (pre-incubation); 24 h (post-irradiation incubation); 72 h (post-irradiation incubation)
-
Result:Reduced SOD2 positive staining to 30% (weak intensity) at 24 h post-irradiation and 50% (weak intensity) at 72 h post-irradiation, while increasing caspase-12 positive staining to 80% (strong intensity) at both 24 h and 72 h post-irradiation in SKOV-3 cells.
Reduced SOD2 positive staining to 25% (moderate intensity) at 24 h post-irradiation, which returned to 100% (weak intensity) at 72 h post-irradiation, while increasing caspase-12 positive staining to 25% (weak-moderate intensity) at 24 h post-irradiation and 100% (strong intensity) at 72 h post-irradiation in MDA MB-231 cells.
Showed minimal caspase-12 staining and full SOD2 staining in unirradiated controls of both cell lines.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 199444-11-6
-
Appearance Solid
-
Molecular Weight 519.55
-
Formula C32H36Cl2N2
-
SMILES
CC1(C)C(/C=C/C2=C(Cl)/C(CCC2)=C/C=C3N(C)C4=C(C=CC=C4)C/3(C)C)=[N+](C)C5=C1C=CC=C5.[Cl-]
-
Synonyms
IR-775 chloride
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Store at room temperature, keep dry and cool
In solvent -80°C 1 year -20°C 6 months
Purity & Documentation
-
Data Sheet (288 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Handling Instructions (2659 KB)
References
[1]. Waszkiewicz M, et al. The photodynamic reaction with IR-775 cyanine combined with 2-methoxyestradiol in ovarian (SKOV-3) and human breast adenocarcinoma (MDA MB-231) cell lines. Photodiagnosis and photodynamic therapy. 2022 Jun;38:102766. [Content Brief]
[2]. Pebam M, et al. IR-775 - Hyptis loaded bioactive nanoparticles for enhanced phyto-photothermal therapy of breast cancer cells. Photodiagnosis and photodynamic therapy. 2023 Dec;44:103872. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
IR-775 chloride Related Classifications
HY-W248590 Related Classifications
Powered by Bioz
Keywords
- IR-775
- 199444-11-6
- IR775
- IR 775
- Fluorescent Dye
- Photosensitizer
- Apoptosis
- Reactive Oxygen Species (ROS)
- SOD
- oxidative stress
- human breast adenocarcinoma (MDA MB-231) cell lines
- human breast adenocarcinoma
- apoptosis
- ovarian carcinoma
- triple-negative breast cancer
- 4T1 cells
- 4T1 3D spheroids
- hyperthermia
- human ovarian adenocarcinoma (SKOV-3) cell lines
- Inhibitor
- inhibitor
- inhibit