Mcl-1-IN-21
Mcl-1-IN-21 is a selective Mcl-1 protein inhibitor. Mcl-1-IN-21 can induce apoptosis, elevate intracellular ROS, reduce mitochondrial membrane potential, exert cytotoxicity against human cervical cancer cells, and inhibit tumor growth in a human cervical cancer xenograft mouse model. Mcl-1-IN-21 can be used for the research of cervical cancer.
For research use only. We do not sell to patients.
- Formula: C39H48BrF3N3O7PS
- Molecular Weight:870.75
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
Mcl-1 |
In Vitro
Mcl-1-IN-21 (compound 9k) (48 h) exhibits potent cytotoxicity against Hela cells (IC50 = 9.55 μM) and moderate activity against MGC-803, HepG2, and LO-2 cells[1].
Mcl-1-IN-21 (5-20 μM; 24 h) induces chromatin condensation in Hela cells at 10 μM and 20 μM after 24 h[1].
Mcl-1-IN-21 (5-20 μM; 24 h) can induce dose-dependent accumulation of ROS in Hela cells after 24 h and reduce mitochondrial membrane potential[1].
Mcl-1-IN-21 (5-20 μM; 24 h) induces concentration-dependent apoptosis in Hela cells after 24 h[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Hela
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Concentration:5 μM, 10 μM, 20 μM
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Incubation Time:24 h
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Result:Induced apoptosis in 11.3%, 17.4%, and 24.4% of Hela cells at 5 μM, 10 μM, and 20 μM, respectively, compared to 6.7% in the control.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/c nude (five-week-old, male) injected with Hela cells[1]
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Dosage:5 mg/kg; 10 mg/kg
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Administration:i.p.; once every other day; 21 days
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Result:Induced dose-dependent tumor growth inhibition with tumor growth inhibition rates of 17% (5 mg/kg) and 21% (10 mg/kg); did not cause obvious body weight loss or adverse effects.
- Mitochondrial membrane-potential fluorescent assay
- Mammalian live/dead viability and cytotoxicity staining
- Annexin V plus membrane-impermeant dye apoptosis staining
- ROS/oxidative-stress fluorescent staining
- Patient-Derived Orthotopic Xenograft (PDOX)
- Cell Viability Determination by MTT Colorimetric Assay
- How to Choose the Right Model Animal
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Cell Cytotoxicity Assay
- Fluorescent plasma-membrane potential dye assay
- Mitochondrial membrane-potential and mitochondrial mass staining
- Patient-Derived Xenograft (PDX)
- Apoptosis Solutions
- Splenic/Portal-Vein Liver Metastasis Xenograft
- Subcutaneous Cell-Line-Derived Xenograft
- Orthotopic Cell-Line Xenograft
- Intraperitoneal/Peritoneal Dissemination Xenograft
Chemical Information
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Molecular Weight 870.75
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Formula C39H48BrF3N3O7PS
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SMILES
O=S(N1C(CCCC(OC2=CC=C(C(P(OCC)(OCC)=O)NC3=CC(Br)=CC=C3)C=C2)=O)C4C5C(CCCN5CCC4)C1)(C6=CC=C(C(F)(F)F)C=C6)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Mcl-1-IN-21 Related Classifications
HY-181054 Related Classifications
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