Mecillinam hydrochloride
Based on 4 publication(s) in Google Scholar
Mecillinam hydrochloride is a β-lactam Antibiotic. Mecillinam hydrochloride inhibits PBP2. Mecillinam hydrochloride exhibits activity against Gram-negative Enterobacteriaceae, and NDM-/OXA-producing E. coli and K. pneumoniae, and can eradicate catheter/bladder mucosa biofilms and clear planktonic bacteria in urine. Mecillinam hydrochloride acts synergistically with β-lactams and Avibactam (HY-14879), enhancing the activity of β-lactams against E. coli in vitro/in vivo. Mecillinam hydrochloride can be used in research on catheter-associated bacteriuria, chlamydial infection and peritonitis/sepsis.
For research use only. We do not sell to patients.
- CAS No.: 32887-08-4
- Formula: C15H24ClN3O3S
- Molecular Weight:361.89
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Protocols
- Purity & Documentation
- References
-
Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Mecillinam hydrochloride
MoreAll Antibiotic Isoforms
More
Biological Activity
Description
IC50 & Target
[6]|
β-lactam |
In Vitro
Mecillinam (0.4-100 μg/mL; 18 h) hydrochloride inhibits a significant proportion of Gram-negative isolates by the agar dilution method, with 75% of Escherichia coli and 62% of Enterobacter inhibited at 6.3 μg/mL[2].
Mecillinam (0.009-10 mg/L; 18 h) hydrochloride inhibits E. coli O54 with an MIC of 10 mg/L and produces complete growth inhibition when used in combination at 0.156 mg/L (FIC 0.016), with a total FIC of 0.046[4].
Mecillinam (0.0625-32 mg/L; 30 min) hydrochloride binds PBP2 with an IC50 of 0.3 mg/L in the E. coli MG1655 PBP competition assay, and Avibactam (HY-14879) does not alter the PBP binding affinity of Mecillinam[5].
Mecillinam (4 mg/L) hydrochloride combined with 1 mg/L Avibactam effectively kills K. pneumoniae isolates KP2, KP5, KP6, KP7, KP8, and ATCC 13883[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Mecillinam (30 mg/kg alone; 2-8 mg/kg in combination with ampicillin; subcutaneous injection; single injection) hydrochloride is ineffective against normal mice with E. coli thigh infection, but low doses strongly enhance the antibacterial activity of Ampicillin (HY-B0522)[4].
Mecillinam (30 mg/kg alone; 2-8 mg/kg in combination with Ampicillin; subcutaneous injection; single injection) hydrochloride is ineffective against Escherichia coli thigh infection in irradiated granulocytopenic mice, but low doses still enhance the antibacterial activity of Ampicillin[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:New Zealand white (male)[1]
-
Dosage:50, 100, 200, or 400 mg/kg body weight each day
-
Administration:intravenous; four divided doses each day; 72 h
-
Result:At sacrifice, log10 cfu of E. coli/mL or cm2 in urethral catheter were NG for 400 mg/kg, 2.02 for 200 mg/kg (2/6), 0.97 for 100 mg/kg (2/5), and 2.53 for 50 mg/kg (2/5).
At 400 mg/kg, at 48 h four of five rabbits had completely eliminated bacteria from urine; at 72 h all rabbits had sterile catheter urine, and urine, catheter, and bladder wall samples were free of detectable bacteria.
-
Animal Model:Swiss-type (SPF, male, 20-25 g)[4]
-
Dosage:30 mg/kg (alone); 2 mg/kg (with ampicillin); 4 mg/kg (with ampicillin); 8 mg/kg (with ampicillin)
-
Administration:subcutaneous; single injection
-
Result:At 30 mg/kg alone, did not produce an antibacterial effect in normal mice.
In combination with ampicillin, produced a strong potentiating effect.
The lowest dose examined in combination was 2 mg/kg.
Potency ratios of the combinations relative to ampicillin alone, plotted against dose in Fig. 5a, increased with dose (2, 4, and 8 mg/kg).
Chemical Information
-
CAS No. 32887-08-4
-
Molecular Weight 361.89
-
Formula C15H24ClN3O3S
-
SMILES
O=C([C@@H](C(C)(C)S[C@]1([H])[C@@H]2/N=C/N3CCCCCC3)N1C2=O)O.Cl
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (4)
-
Journal Impact Factor
-
Most Recent
-
J Antimicrob Chemother
Unravelling the triad of penicillin-binding proteins, β-lactamase activity, and mRNA dynamics in Pseudomonas aeruginosa AmpC induction. [Abstract]2025 Nov 5:dkaf408. PMID: 41206063 -
Microbiol Spectr
Penicillin-Binding Protein Occupancy Dataset for 18 β-Lactams and 4 β-Lactamase Inhibitors in Neisseria gonorrhoeae. [Abstract]2023 Jun 15;11(3):e0069223. PMID: 37093051 -
Microbiol Spectr
PBP Target Profiling by β-Lactam and β-Lactamase Inhibitors in Intact Pseudomonas aeruginosa: Effects of the Intrinsic and Acquired Resistance Determinants on the Periplasmic Drug Availability. [Abstract]2023 Feb 14;11(1):e0303822. PMID: 36475840 -
J Glob Antimicrob Resist
Susceptibility of multidrug-resistant Escherichia coli and Klebsiella pneumoniae to oral antibiotics in Australia. [Abstract]2025 Aug 8:44:435-441. PMID: 40784378
Solvent & Solubility
In Vitro:
DMSO : 50 mg/mL (138.16 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
References
[1]. Olson ME, et al. Amdinocillin treatment of catheter-associated bacteriuria in rabbits. The Journal of infectious diseases. 1989 Jun;159(6):1065-72. [Content Brief]
[2]. Neu HC, et al. Mecillinam, a novel penicillanic acid derivative with unusual activity against gram-negative bacteria. Antimicrobial agents and chemotherapy. 1976 May;9(5):793-9. [Content Brief]
[5]. List KK, et al. Synergy between Mecillinam and Ceftazidime/Avibactam or Avibactam against Multi-Drug-Resistant Carbapenemase-Producing Escherichia coli and Klebsiella pneumoniae. Antibiotics (Basel). 2022 Sep 20;11(10):1280. [Content Brief]
Complete Stock Solution Preparation Table
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7633 mL | 13.8164 mL | 27.6327 mL | 69.0818 mL |
| 5 mM | 0.5527 mL | 2.7633 mL | 5.5265 mL | 13.8164 mL | |
| 10 mM | 0.2763 mL | 1.3816 mL | 2.7633 mL | 6.9082 mL | |
| 15 mM | 0.1842 mL | 0.9211 mL | 1.8422 mL | 4.6055 mL | |
| 20 mM | 0.1382 mL | 0.6908 mL | 1.3816 mL | 3.4541 mL | |
| 25 mM | 0.1105 mL | 0.5527 mL | 1.1053 mL | 2.7633 mL | |
| 30 mM | 0.0921 mL | 0.4605 mL | 0.9211 mL | 2.3027 mL | |
| 40 mM | 0.0691 mL | 0.3454 mL | 0.6908 mL | 1.7270 mL | |
| 50 mM | 0.0553 mL | 0.2763 mL | 0.5527 mL | 1.3816 mL | |
| 60 mM | 0.0461 mL | 0.2303 mL | 0.4605 mL | 1.1514 mL | |
| 80 mM | 0.0345 mL | 0.1727 mL | 0.3454 mL | 0.8635 mL | |
| 100 mM | 0.0276 mL | 0.1382 mL | 0.2763 mL | 0.6908 mL |
Mecillinam hydrochloride Related Classifications
HY-A0269A Related Classifications
Powered by Bioz