MIH-36
MIH-36 is a VEGFR-2 modulator and anticancer agent. MIH-36 decreases VEGFR-2 surface expression in cells while upregulating VEGFR-2 mRNA. MIH-36 induces Apoptosis. MIH-36 exhibits selective anticancer activity against luminal and triple-negative breast cancer. MIH-36 can be used for research on breast cancer.
For research use only. We do not sell to patients.
- Formula: C21H13F6N5O4S
- Molecular Weight:545.41
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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All VEGFR Isoforms
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Biological Activity
Description
IC50 & Target
[1]|
VEGFR2 |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| MDA-MB-231 | IC50 |
18.9 μM
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Antiproliferative activity against human breast cancer MDA-MB-231 cells assessed as viability reduction incubated 48 hrs by MTT assay.
Antiproliferative activity against human breast cancer MDA-MB-231 cells assessed as viability reduction incubated 48 hrs by MTT assay.
|
42759396 |
| MCF7 | IC50 |
13.74 μM
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Antiproliferative activity against human breast cancer MCF-7 cells assessed as viability reduction incubated 48 hrs by MTT assay.
Antiproliferative activity against human breast cancer MCF-7 cells assessed as viability reduction incubated 48 hrs by MTT assay.
|
42759396 |
| L929 | IC50 |
49.40 μM
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Cytotoxicity against mouse L929 fibroblasts assessed as viability reduction incubated 48 hrs by MTT assay.
Cytotoxicity against mouse L929 fibroblasts assessed as viability reduction incubated 48 hrs by MTT assay.
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42759396 |
In Vitro
MIH-36 (48 h) inhibited scratch wound closure in MCF-7 cells compared with the control[1].
MIH-36 (48 h) inhibited scratch wound closure in MDA-MB-231 compared with the control[1].
MIH-36 (48 h) reduced the percentage of VEGFR-2 surface-positive cells in MDA-MB-231 (80.4%) and MCF-7 (32.9%) and altered the MFI, indicating modulation of surface VEGFR-2[1].
MIH-36 (48 h) upregulates VEGFR-2 mRNA in MDA-MB-231 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MCF-7, MDA-MB-231, and L929 cells
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Concentration:400, 200, 100, 50, 25, and 12.5 μM
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Incubation Time:48 h (MIH-36); 4 h (MTT)
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Result:MIH-36 IC50 values were 18.9 μM in MDA-MB-231 cells, 13.74 μM in MCF-7 cells, and 49.40 μM in L929 cells.
MIH-36 selectivity indices were 2.61 for MDA-MB-231 and 3.59 for MCF-7.
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Cell Line:MDA-MB-231 and MCF-7 cells
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Concentration:IC50
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Incubation Time:48 h
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Result:In MDA-MB-231 cells, MIH-36 decreased live cells to Q4 68.6% and increased late apoptotic cells to Q2 24.7%.
In MCF-7 cells, MIH-36 increased late apoptotic cells to Q2 26.1%, decreased live cells to Q4 59.7%, with Q3 11.7% and Q1 2.49%.
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Cell Line:MDA-MB-231 cells
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Concentration:Various concentrations
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Incubation Time:48 h (MIH-36); PCR cycling as described
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Result:Significantly increased VEGFR-2 mRNA expression compared with both the control group and the DMSO group.
Chemical Information
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Molecular Weight 545.41
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Formula C21H13F6N5O4S
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SMILES
S=C(NC1=CC=C(C(N/N=C/C2=CC=C([N+]([O-])=O)O2)=O)C=C1)NC3=CC(C(F)(F)F)=CC(C(F)(F)F)=C3
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
MIH-36 Related Classifications
HY-189691 Related Classifications
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