N-Acetylhistidine monohydrate
Based on 1 Customer Validation
N-Acetylhistidine monohydrate is a compatible osmolyte in the brain, retina, and lens of poikilothermic vertebrates, and binds to E. coli HisJ with a Kd of 27 μM. N-Acetylhistidine monohydrate participates in the regulatory volume decrease process under hypotonic stress, assists cells in restoring homeostatic volume, and acts as a gradient-driven molecular water pump, exerting a protective effect against hypotonic swelling. N-Acetylhistidine monohydrate can be used in combination with glutamate to inhibit the cobalt-resistant postsynaptic component in the electroretinogram. N-Acetylhistidine monohydrate reduces Ca2+-induced growth inhibition and restores cell growth. N-Acetylhistidine monohydrate can be used in research related to cataracts, diabetic distal symmetric polyneuropathy, and stroke.
For research use only. We do not sell to patients.
- Purity : 99.96%
- CAS No.: 39145-52-3
- Formula: C8H13N3O4
- Molecular Weight:215.21
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
In Vitro
N-Acetylhistidine monohydrate (24 h) efflux is stimulated by hypotonic exposure in cultured Atlantic salmon lenses[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 39145-52-3
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Appearance Solid
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Molecular Weight 215.21
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Formula C8H13N3O4
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Color White to off-white
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SMILES
O=C(O)[C@H](CC1=CNC=N1)NC(C)=O.[H]O[H]
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
H2O : 62.5 mg/mL (290.41 mM; ultrasonic and warming and heat to 60°C)
DMSO : 20 mg/mL (92.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2 mg/mL (9.29 mM); Clear solution
This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2 mg/mL (9.29 mM); Clear solution
This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 12.5 mg/mL (58.08 mM); Clear solution; Need ultrasonic
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (290 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 4.6466 mL | 23.2331 mL | 46.4662 mL | 116.1656 mL |
| 5 mM | 0.9293 mL | 4.6466 mL | 9.2932 mL | 23.2331 mL | |
| 10 mM | 0.4647 mL | 2.3233 mL | 4.6466 mL | 11.6166 mL | |
| 15 mM | 0.3098 mL | 1.5489 mL | 3.0977 mL | 7.7444 mL | |
| 20 mM | 0.2323 mL | 1.1617 mL | 2.3233 mL | 5.8083 mL | |
| 25 mM | 0.1859 mL | 0.9293 mL | 1.8586 mL | 4.6466 mL | |
| 30 mM | 0.1549 mL | 0.7744 mL | 1.5489 mL | 3.8722 mL | |
| 40 mM | 0.1162 mL | 0.5808 mL | 1.1617 mL | 2.9041 mL | |
| 50 mM | 0.0929 mL | 0.4647 mL | 0.9293 mL | 2.3233 mL | |
| 60 mM | 0.0774 mL | 0.3872 mL | 0.7744 mL | 1.9361 mL | |
| 80 mM | 0.0581 mL | 0.2904 mL | 0.5808 mL | 1.4521 mL | |
| H2O | 100 mM | 0.0465 mL | 0.2323 mL | 0.4647 mL | 1.1617 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
N-Acetylhistidine monohydrate Related Classifications
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