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Cat. No.: HY-120420

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N4Py

Name: N4Py
Brand: MedChemExpress
SKU: HY-120420
Price: 200.00 USD
Availability: InStock
Rating: 5 (1 reviews)

N4Py is a bleomycin-mimetic pentadentate ligand that targets intracellular FeII and reversibly chelates a variety of transition metals. N4Py chelates induce apoptosis through two pathways: DNA cleavage via ROS generation and XIAP downregulation via ZnII chelation, with only FeII/FeIII-N4Py exhibiting potent DNA cleavage activity. The folate conjugate of N4Py targets folate receptors, can be intracellularly cleaved, and selectively kills FR-positive cancer cells. N4Py can be used in research on various cancers including nasopharyngeal epidermoid carcinoma, ovarian cancer, and mammary adenocarcinoma.

For research use only. We do not sell to patients.

CAS No.: 167695-87-6
Formula: C₂₃H₂₁N₅
Molecular Weight:367.45

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (289 KB)

N4Py Chemical Structure

CAS No.: 167695-87-6

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
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- In Vitro
Chemical Information
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Biological Activity

Description

All IAP Isoforms :

In Vitro

Anti-cancerApoptosisOxidative StressOthers

Anti-cancer

Mn (II)-N4Py, Fe (II)-N4Py and Fe (III)-N4Py formed by N4Py significantly reduce the metabolic activity of A2780, SKOV3 and OSE-C2 cells, with IC₅₀ values of 5-10 μM^[1].

Anti-cancer

Apoptosis

Mn (II)-N4Py, Fe (II)-N4Py and Fe (III)-N4Py induce significant late apoptosis/necrotic cell death in A2780, SKOV3 and OSE-C2 cells, among which free N4Py exhibits the strongest cytotoxicity, while Cu (II)-N4Py and Zn (II)-N4Py show minimal cytotoxicity^[1].

Oxidative Stress

Mn (II)-N4Py, Fe (II)-N4Py, Fe (III)-N4Py, and Cu (II)-N4Py induce hROS production in A2780 cells, while Zn (II)-N4Py has no such effect; NAC abrogates hROS production induced by all these substances except Cu (II)-N4Py, and instead enhances hROS production induced by Cu (II)-N4Py^[1].

Others

N4Py can form Fe (II)-N4Py and Fe (III)-N4Py, both of which efficiently cleave supercoiled pUC18 plasmid DNA under cell-free conditions^[1].
N4Py forms complexes with Fe²⁺, Fe³⁺, Cu²⁺, Mn²⁺, and Zn²⁺ that differ in spectral and electrochemical properties, and Fe²⁺-N4Py exhibits characteristic metal-to-ligand charge transfer absorption^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Related Protocols :

Chemical Information

CAS No. 167695-87-6
Molecular Weight 367.45
Formula C₂₃H₂₁N₅
SMILES

C1(C(C2=CC=CC=N2)N(CC3=CC=CC=N3)CC4=NC=CC=C4)=NC=CC=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (289 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Geersing A, et al. Importance of Metal-Ion Exchange for the Biological Activity of Coordination Complexes of the Biomimetic Ligand N4Py. Inorganic chemistry. 2018 Jul 02;57(13):7748-7756. [Content Brief]

[2]. Geersing A. Redox-active N4Py-metal complexes in human cell cultures. Groningen: Rijksuniversiteit Groningen, 2017. ISBN 978-90-367-9760-3.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

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100 mg

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