NF-κB-IN-21
NF-κB-IN-21 is a NF-κB signaling pathway inhibitor. NF-κB-IN-21 reduces the phosphorylation levels of IκBα and p65, and inhibits the nuclear translocation of p65. NF-κB-IN-21 downregulates the expression of iNOS, inhibits nitric oxide production, and decreases the levels of pro-inflammatory cytokines TNF-α and IL-6. NF-κB-IN-21 is used in the research of inflammatory diseases.
For research use only. We do not sell to patients.
- Formula: C32H44O6S
- Molecular Weight:556.75
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
iNOS |
IL-6 |
In Vitro
NF-κB-IN-21 (0.041-0.370 μM; 24 h co-incubation with LPS) inhibits LPS-induced activation of the NF-κB pathway in RAW264.7 cells in a dose-dependent manner by reducing the phosphorylation levels of IκBα and p65, and downregulates the expression of downstream iNOS[1].
NF-κB-IN-21 inhibits LPS-induced nuclear translocation of p65 in RAW264.7 cells, confirming its inhibitory effect on the activation of the NF-κB pathway[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:LO2 human normal hepatocytes
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Concentration:0.005-3.333 μM
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Incubation Time:24 h, 72 h
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Result:Showed no significant cytotoxicity across all tested concentrations after 24 h treatment.
Exhibited dose-dependent cytotoxicity with an IC50 value of 6.557×10-7 M after 72 h treatment, comparable to Celastrol's IC50 of 7.037×10-7 M.
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Cell Line:RAW264.7 murine macrophage cells
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Concentration:0.014-0.123 μM
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Incubation Time:1 h pretreatment, followed by 24 h LPS co-incubation
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Result:Dose-dependently inhibited LPS-induced IL-6 production, with near-complete inhibition at 0.123 μM, and significant inhibition at 0.041 μM.
Inhibited TNF-α production only at 0.123 μM, with no significant effect at 0.041 or 0.014 μM.
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Cell Line:RAW264.7 murine macrophage cells
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Concentration:0.041-0.370 μM
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Incubation Time:24 h co-incubation with LPS
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Result:Dose-dependently reduced LPS-induced phosphorylation of IκBα and p65, with significant inhibition observed at 0.123 μM and 0.041 μM.
Dose-dependently downregulated LPS-induced iNOS expression, consistent with NO inhibition results.
Left total p65 and IκBα levels unchanged across treatment groups.
Chemical Information
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Molecular Weight 556.75
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Formula C32H44O6S
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SMILES
OC1=C(C2C(SCC(OC)=O)C=C3[C@](C)([C@]4(CC[C@]3(C2=CC1=O)C)C)CC[C@@]5(C)CC[C@@](C(O)=O)(C[C@]54[H])C)C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
NF-κB-IN-21 Related Classifications
HY-184716 Related Classifications
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