Cat. No.: HY-114849

Norflurazon

Name: Norflurazon
Brand: MedChemExpress
SKU: HY-114849
Price: 70.00 USD
Availability: InStock
Rating: 5 (1 reviews)

(Synonyms: SAN 9789)

Customer Review

Based on 1 Customer Validation

Norflurazon (SAN 9789) is a pre-emergence Herbicide. Norflurazon non-competitively inhibits phytoene desaturase by competing with the enzyme cofactor and disrupts carotenoid biosynthesis, thereby leading to chlorophyll photodegradation and chlorosis. Norflurazon inhibits MGDG synthase, CDP-choline phosphotransferase, Omega-3 FAD7 desaturase, and PG delta-3-trans desaturase, and activates LysoPC-acyltransferase and Omega-3 FAD3 desaturase. Norflurazon inhibits photosynthetic membrane organization, mitochondrial respiration, ATP production, PI3K signaling, and ERK1/2 phosphorylation. Norflurazon activates MAPK P38 phosphorylation and ER stress signaling. Norflurazon induces morphological malformations and cardiovascular effects in zebrafish embryos.

For research use only. We do not sell to patients.

Purity : 99.94%
CAS No.: 27314-13-2
Formula: C₁₂H₉ClF₃N₃O
Molecular Weight:303.67

Storage:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

Norflurazon Chemical Structure

CAS No.: 27314-13-2

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

^[4]

ERK1

ERK2

All PI3K Isoforms :

All ERK Isoforms :

All p38 MAPK Isoforms :

In Vitro

Bacterial InfectionCell Cycle RegulationApoptosisMitochondrial FunctionOthers

Bacterial Infection

Norflurazon (0.1-500 μg/plate) is negative for genotoxicity in the Salmonella typhimurium and Saccharomyces cerevisiae D4 reverse gene mutation test^[3].
Norflurazon (5-5,000 μg/plate) is negative for genotoxicity in the Salmonella typhimurium and Escherichia coli reverse gene mutation test^[3].
Norflurazon (500-10,000 μg/disc) is negative in the Bacillus subtilis Rec assay^[3].

Cell Cycle Regulation

Norflurazon (0-100 μM; 48 h) induces cell cycle arrest in pTr and pLE cells, increasing the sub-G₁ population by 1.44-fold and 1.35-fold, respectively, at 100 μM^[4].

Apoptosis

Norflurazon (0-100 μM; 48 h) induces apoptosis in pTr and pLE cells, with a significant 154% increase in apoptosis observed in pLE cells at 100 μM^[4].

Mitochondrial Function

Norflurazon (0-100 μM; 48 h) induces mitochondrial dysfunction in pTr and pLE cells, increasing the JC-1 monomer percentage by 207% and 203%, respectively, at 100 μM^[4].

Others

Norflurazon (100 μM; two weeks) inhibits CDP-choline phosphotransferase activity while activating lysoPC-acyltransferase activity in soybean leaf microsomal membrane fractions^[1].
Norflurazon (100 μM; two weeks) inhibits MGDG synthase activity in soybean leaf chloroplastic membrane fractions^[1].
Norflurazon (0-10 µM; 96 h) causes concentration-dependent chlorophyll bleaching, with 4 A⁺ most affected after 96 h^[2].
Norflurazon (0-100 μM; 48 h) suppresses proliferation of pTr and pLE cells, with 100 μM reducing proliferation by 53% and 68%, respectively^[4].
Norflurazon (0-100 μM; 48 h) increases intracellular calcium levels in pTr and pLE cells, with 100 μM causing a 133% and 141% increase, respectively^[4].
Norflurazon (0-100 μM) regulates cell proliferation and growth through the PI3K/MAPK signaling pathways in pTr and pLE cells, activating P38 and inactivating AKT and P70S6K^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[4]

Cell Line:

porcine trophectoderm (pTr) and porcine uterine luminal epithelial (pLE) cells
Concentration:

0, 20, 50, or 100 μM
Incubation Time:

48 h
Result:

Decreased cell proliferation at various concentrations.
Reduced proliferation of pTr and pLE cells by 53% and 68%, respectively, at 100 μM.

Cell Cycle Analysis^[4]

Cell Line:

pTr and pLE cells
Concentration:

0, 20, 50, or 100 μM
Incubation Time:

48 h
Result:

Increased the sub-G1 cell population by 1.44-fold and 1.35-fold for pTr and pLE cells, respectively, at 100 μM.

Apoptosis Analysis^[4]

Cell Line:

pTr and pLE cells
Concentration:

0, 20, 50, or 100 μM
Incubation Time:

48 h
Result:

Increased apoptosis of 119% for pTr cells was not significant while the 154% increase for pLE cells was significant at 100 μM.

In Vivo

Local Vascular RegulationOthers

Local Vascular Regulation

Norflurazon (50-100 μM; in embryo medium; daily; 6 h post-fertilization to 96 h post-fertilization) causes developmental defects in zebrafish including reduced hatching rate, shortened body length, yolk sac enlargement, pericardial edema, disrupted brain vasculature, and impaired cardiac looping, with cardiovascular toxicity and apoptosis observed at concentrations of 50 and 100 μM^[5].

Others

Norflurazon (85-340 ppm; dietary; 18 weeks) did not adversely affect fertility, reproductive parameters or pup development in mice at the tested dietary concentrations of 85, 170, or 340 ppm^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

CD-1 (HaM/ICR Swiss) (male and female)^[3]
Dosage:

85, 170, 340 ppm
Administration:

dietary; 18 weeks
Result:

The reproductive portion of the study did not reveal any indications of adverse effects of the test substance on fertility, reproductive parameters or pup development.

Animal Model:

wild-type AB strain and transgenic flk1:eGFP line (embryos exposed from 6 h post-fertilization)^[5]
Dosage:

50 μM; 100 μM
Administration:

in embryo medium; solution replaced daily; 6 h post-fertilization to 96 h post-fertilization
Result:

Increased the proportion of unhatched live embryos, with hatching rates of 19.4% at 50 μM and 11.1% at 100 μM at 72 hpf compared to 86.1% in controls.
Reduced the relative total body length to 82.1% at 100 μM.
Enlarged the yolk sac size at 50 and 100 μM.
Induced pericardial edema at 100 μM.
Reduced brain vasculature density to 69.1% at 50 μM and 32.7% at 100 μM.
Increased the distance between sinus venosus and bulbus arteriosus at 100 μM, indicating impaired cardiac looping.
Increased fluorescence intensity 1.2-fold at 50 μM and 1.5-fold at 100 μM.

Related Protocols :

Chemical Information

CAS No. 27314-13-2
Appearance Solid
Molecular Weight 303.67
Formula C₁₂H₉ClF₃N₃O
Color Off-white to light yellow
SMILES

O=C1C(Cl)=C(C=NN1C2=CC=CC(C(F)(F)F)=C2)NC
Synonyms

SAN 9789
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (411.63 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.2930 mL

16.4652 mL

32.9305 mL

5 mM

0.6586 mL

3.2930 mL

6.5861 mL

10 mM

0.3293 mL

1.6465 mL

3.2930 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight Molecular Weight

Dilution Calculator

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (289 KB)
SDS (458 KB)
- English - EN (458 KB)
- Français - FR (458 KB)
- Deutsch - DE (458 KB)
- Norwegian - NO (458 KB)
- Español - ES (458 KB)
- Swedish - SV (458 KB)
- Italian - IT (458 KB)
- Korean - KR (458 KB)
- Portuguese - PT (458 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Abrous-Belbachir O, et al. Evidence that norflurazon affects chloroplast lipid unsaturation in soybean leaves (Glycine max L.). Journal of agricultural and food chemistry. 2009 Dec 09;57(23):11434-40. [Content Brief]

[2]. Leetanasaksakul K, et al. Proteomic analysis of differential responses to norflurazon herbicide in the model green alga Chlamydomonas reinhardtii. Scientific reports. 2025 Aug 27;15(1):31601.

[3]. Niemann L, et al. First draft prepared by Lars Niemann1 and Alan Boobis2 Department of Pesticides Safety, German Federal Institute for Risk Assessment, Berlin, Germany 2 Department of Medicine, Imperial College London, London, United Kingdom[J].

[4]. Hong T, et al. Norflurazon causes cell death and inhibits implantation-related genes in porcine trophectoderm and uterine luminal epithelial cells. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 2024 Apr;186:114559. [Content Brief]

[5]. An G, et al. Norflurazon causes developmental defects including cardiovascular abnormalities in early-stage zebrafish (Danio rerio)[J]. Journal of Animal Reproduction and Biotechnology, 2022, 37(3): 176-182.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.2930 mL	16.4652 mL	32.9305 mL	82.3262 mL
	5 mM	0.6586 mL	3.2930 mL	6.5861 mL	16.4652 mL
	10 mM	0.3293 mL	1.6465 mL	3.2930 mL	8.2326 mL
	15 mM	0.2195 mL	1.0977 mL	2.1954 mL	5.4884 mL
	20 mM	0.1647 mL	0.8233 mL	1.6465 mL	4.1163 mL
	25 mM	0.1317 mL	0.6586 mL	1.3172 mL	3.2930 mL
	30 mM	0.1098 mL	0.5488 mL	1.0977 mL	2.7442 mL
	40 mM	0.0823 mL	0.4116 mL	0.8233 mL	2.0582 mL
	50 mM	0.0659 mL	0.3293 mL	0.6586 mL	1.6465 mL
	60 mM	0.0549 mL	0.2744 mL	0.5488 mL	1.3721 mL
	80 mM	0.0412 mL	0.2058 mL	0.4116 mL	1.0291 mL
	100 mM	0.0329 mL	0.1647 mL	0.3293 mL	0.8233 mL

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Norflurazon

All PI3K Isoforms

All ERK Isoforms

All p38 MAPK Isoforms