Oxythiamine chloride
Based on 3 publication(s) in Google Scholar
Oxythiamine (Hydroxythiamin) chloride, an analogue of anti-metabolite, can suppress the non-oxidative synthesis of ribose and induce cell apoptosis. Oxythiamine chloride is a thiamine antagonist and inhibits transketolase (TK). Oxythiamine chloride inhibits cancer cell apoptosis and inhibits cell proliferation.
For research use only. We do not sell to patients.
- CAS No.: 582-36-5
- Formula: C12H16ClN3O2S
- Molecular Weight:301.79
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Publications Citing Use of MedChemExpress (MCE) Oxythiamine chloride
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Customer Validation & Images
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Cell Proliferation/Viability Assay
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WB
All Endogenous Metabolite Isoforms
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Biological Activity
Description
IC50 & Target
Vitamin B1, Thiamine, Transketolase[1].
In Vitro
Oxythiamine (0-40 μM, 2 days) chloride inhibits cell viability of MIA PaCa-2 cells (IC50: 14.95 μM)[1].
Oxythiamine (0-500 μM, 48 h) chloride suppresses expression of 14-3-3 protein beta/alpha in MIA PaCa-2 cells[1].
Oxythiamine (0.1-100 μM, 6-48 h) chloride inhibits A549 cell proliferation[3].
Oxythiamine (0.1-100 μM, 24 h) chloride induces A549 cell apoptosis[3].
Oxythiamine (0-20 μM) chloride inhibits the invasion and migration (IC50: 8.75 μM) of Lewis lung carcinoma (LLC) cells[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MIA PaCa-2 cells
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Concentration:0-40 μM
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Incubation Time:2 days
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Result:Inhibited cell viability with an IC50 of 14.95 μM.
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Cell Line:MIA PaCa-2 cells
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Concentration:0, 5, 50, 500 μM
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Incubation Time:48 h
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Result:Inhibited 14-3-3 protein beta/alpha expression, and increased alpha-enolase.
In Vivo
Oxythiamine (250 or 500 mg/kg, daily for 5 week) chloride inhibits tumor cell metastasis via inhibition of MMPs in mice implanted (s.c.) with LLC cells[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Ehrlich's ascites tumor hosting mice[2]
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Dosage:100-500 mg/kg
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Administration:i.p., 4 days
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Result:Inhibited tumor growth by 43% at 300 mg/kg and 84% at 500 mg/kg.
- Annexin V plus membrane-impermeant dye apoptosis staining
- BrdU Incorporation Assay
- Somatic Cell Culture
- CFSE Dye Dilution Proliferation Assay
- Dye-dilution cell tracking and proliferation staining
- CCK-8/WST-8 Cell Proliferation Assay
- Protocol for Cell Cycle
- Cell Counting-Based Growth Curve Assay
- Colony Formation (Clonogenic) Assay
- Cell Viability Determination by MTT Colorimetric Assay
- EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Ki-67 Immunostaining Proliferation Assay
- PCNA Immunodetection Proliferation Assay
- Protocol for Cell Counting and Cell Density Analysis
- Apoptosis Solutions
- MTT Cell Proliferation Assay
Chemical Information
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CAS No. 582-36-5
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Molecular Weight 301.79
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Formula C12H16ClN3O2S
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SMILES
CC1=C(CCO)SC=[N+]1CC2=CN=C(C)NC2=O.[Cl-]
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Synonyms
Hydroxythiamine chloride
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (3)
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Journal Impact Factor
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Most Recent
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Nat Commun
2022 Oct 17;13(1):6121. PMID: 36253417
Oxythiamine chloride purchased from MedChemExpress. Usage Cited in: Nat Commun. 2022 Oct 17;13(1):6121. [Abstract]
Treatment with TKT inhibitor Oxythiamine (OT, 20 μM) abolished SIRT5-induced protection from DNA damage.
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Acta Physiol
Transketolase drives the development of aortic dissection by impairing mitochondrial bioenergetics. [Abstract]2024 Apr;240(4):e14113. PMID: 38380737 -
PLoS Pathog
White spot syndrome virus IE1 protein hijacks the host pentose phosphate pathway to fuel viral replication. [Abstract]2026 Jan 27;22(1):e1013913. PMID: 41592137
Oxythiamine chloride purchased from MedChemExpress. Usage Cited in: PLoS Pathog. 2026 Jan 27;22(1):e1013913. [Abstract]
Effects of PPP enzyme inhibitors on WSSV infection. Shrimp were injected with 2 μM oxythiamine (OT; TKTL2 inhibitor), 20 μM 6-aminonicotinamide (6-AN; G6PD inhibitor), or DMSO control. Cytotoxicity was evaluated by CCK 8 assay.
Purity & Documentation
References
[1]. Wang J, et al. Inhibition of transketolase by oxythiamine altered dynamics of protein signals in pancreatic cancer cells. Exp Hematol Oncol. 2013 Jul 27;2:18. [Content Brief]
[2]. Raïs B, et al. Oxythiamine and dehydroepiandrosterone induce a G1 phase cycle arrest in Ehrlich's tumor cells through inhibition of the pentose cycle. FEBS Lett. 1999 Jul 30;456(1):113-8. [Content Brief]
[3]. Bai L, et al. A dose- and time-dependent effect of oxythiamine on cell growth inhibition in non-small cell lung cancer. Cogn Neurodyn. 2022 Jun;16(3):633-641. [Content Brief]
[4]. Yang CM, et al. The in vitro and in vivo anti-metastatic efficacy of oxythiamine and the possible mechanisms of action. Clin Exp Metastasis. 2010 May;27(5):341-9. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Oxythiamine chloride Related Classifications
HY-107430B Related Classifications
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